From "Fei Chang Disease" Model Of Rat SP,VIP Content Of "the Lung And The Large Intestine Being Interior-exteriorly Related"

Objective:To establish "lung disease"(allergic asthma),"bowel disease"(constipation) and "Fei Chang disease"(allergic asthma and constipation) rats pathological models, and observe liver, heart and lung, spleen, stomach, kidney and other organs with pathological changes of gastrointestinal tissues and correlation,and its related substances SP, VIP expression and association,and then explore the relationship between the lung and the biological mechanisms of colorectal table.Methods:This study selected 40 Wistar rats, SPF grade, male. Adaptive feeding 7 days were randomly divided into the control of blank group,"lung disease" group,"bowel disease" group and "Fei Chang disease" group,10 in each group. "lung disease" group will OVA lmg and AL (OH)3 200mg lml normal saline gel in the preparation of sensitizing agent, on the modeling day 1 and 7 days in rats with bilateral chest, groin subcutaneous injection 0.15ml.The sensitization and intraperitoneal injection of 0.4ml, a total of 1ml.The 14th day of modeling the rats placed in plexiglass boxes, ultrasonic nebulized 1% OVA induced asthma, day 1, every 30min, a total of lw."bowel disease" group received diphenoxylate compound orally, the dose 10mg/kg, every other day 1,a total of 11 times. "Fei Chang disease" group with OVA1mg and AL(OH)3 200mg 1ml normal saline gel in the preparation of sensitization, in modeling the 0 and 7 days injection of allergenic agents, modeling the first 14 days starting spray once a day; the same time, given the lung disease group co-intestinal diphenoxylate compound tablets gavage dose of lOmg/kg, every other day, a total of 11 times. Rats were observed during the experiment in general.20 days of experiment, after atomization, the rats were sacrificed, the organs and tissues by light microscopy observation of changes in HE staining, electron microscopic ultrastructural changes of internal organs; and detected by immunohistochemistry organs and tissues, and SP,VIP expression.Results:1.Light microscope: The control of blank group:structural integrity of lung tissue, no congestion and inflammatory cell infiltration, structural integrity of colonic mucosa, muscularis, serosa was no infiltration of inflammatory cells."Lung disease" group:widened alveolar septum in lung tissue, capillary congestion, inflammatory cell infiltration around blood vessels; rat colon serosal inflammatory cells infiltration."Bowel disease" group:a small amount of lung tissue inflammatory cell infiltration around capillaries; rat colonic mucosa, the muscular, serosal infiltration of inflammatory cells."Fei Chang disease" group:rat lungalveolar Interval widened, blood vessel wall tissue around a large number of interstitiallung infiltration of inflammatory cells, rat colon muscularis, serosal infiltration of inflammatory cells.Rats liver, heart, spleen, kidney and stomach, duodenum, jejunum, ileum, rectal tissue biopsy found no significant changes.2.Electron microscope:The control of blank group:the normal lung tissue structure, mitochondria without swelling, glands normal colon tissue, mucous membranes, no edema, no swelling of mitochondria, closely aligned intestinal micro-villi."Lung disease" group:rat lung tissue of typeâ…¡cells swelling of mitochondria; rat colon microvilli appear."Bowel disease" group:rat lung typeâ…¡cells appeared slightly swollen mitochondria; rat colon tissue of microvilli, mitochondrial swelling."Fei Chang disease" group:rat lung Typeâ…¡cells appear obvious swelling of mitochondria, rat colon glandatrophy, submucosal edema, mitochondrial swelling, intestinal disorders sparsearrangement of micro-villi of different lengths.3.Heart, liver, spleen,lung and kidney and gastrointestinal tissue SP,VIP:"Lung disease" group,"bowel disease" group and "Fei Chang disease" group rat heart, liver, spleen, kidney and stomach, duodenum, jejunum, ileum, rectum SP, VIP expression compared with the control group no significant difference(P>0.05). Compared with the control group, "lung disease" group decreased expression of rat colon SP, VIP expression increased, showed a difference (P<0.05);compared with the control group, "bowel disease" group SP expression in lung tissue increased VIP expression decreased or showed a significant difference between (P<0.05orP<0.01); compared with the control group, "Fei Chang disease" group lung tissue increased expression of SP, VIP expression decreased SP expression in colon lower, VIP expression increased, showed a significant difference (P<0.01); and "lung disease" group, "Fei Chang disease" group decreased expression of rat colon SP, VIP expression increased, showed a difference (P<0.05); and "bowel disease" group more, "Fei Chang disease" group lung tissue increased expression of SP, VIP was decreased, showed a significant difference or differences (P<0.05orP<0.01).Conclusions:1.Pathological changes of lung and colon relevance and specificity:experimental study found that "bowel disease" state, the rat heart, liver, spleen, kidney and other tissues had no significant pathological changes, there is only pathological changes in lung tissue; "lung disease" state, the rat stomach, second Duodenum, jejunum, ileum, rectum no obvious morphological changes, pathological changes in colon tissue only more obvious, and in the "Fei Chang disease" state, the lung tissue and pathological changes in colon tissue than "lung disease" group, "bowel disease" group is more evident, suggesting that in the lung and intestine, bowel and lung association, lung and colon the most relevant.2.Lung and gastrointestinal tissue SP,VIP expression in correlation with the specific:experimental study found that "bowel disease" state, the rat heart, liver, spleen, kidney and other tissues had no significant pathological changes, there is only pathological changes in lung tissue; "lung disease" state, the rat stomach, second Duodenum, jejunum, ileum, rectum no obvious morphological changes, pathological changes in colon tissue only more obvious, and in the "Fei Chang disease" state, the lung tissue and pathological changes in colon tissue than "bowel disease"group, "bowel disease"group is more evident, suggesting that in the lung and intestine, bowel and lung association, lung and colon the most relevant.