Expression And Clinic Significance Of HSP Gp96 And C-jun And CEA In Colorectal Carcinoma

Objective To make clear the roles which HSP gp96 and c‐jun and CEA played in colorectal carcinoma and their relationship, we intended to detect the expression of them and analyzed the relationship among them. we hope to know the relationship between their expression and clinic significance in colorectal cancer.Methods (1) SP immunohistochemical method was applied to detect the expression of HSP gp96 and c‐jun and CEA in 78 cases of colorectal cancer and corresponding paracancer tissues. (2) Analyse the relationship between expression of HSP gp96 and c‐jun and CEA and the development,differentiation degree,pathological pattern,clinical staging,lymph node metastasis and prognosis of colorectal cancer. (3) All data were processed by SPSS 13.0.Results (1) Expression of HSP gp96 Positive expression of HSP gp96 in colorectal cancer was obviously higher than that in paracancer tissue(χ~2 =59.116, P<0.001); HSPgp96 positive expression in poorly differentiated cancer type was higher than that in moderately and well differentiated type(χ~2 =5.087, P=0.025); The expression rate in positive lymph nodes was higher than that of negative lymph nodes(χ~2 =9.665, P=0.002); Positive HSP gp96 expression of B stage + C stage of Dukes classification was higher than that of A stage + C stage(χ~2 =5.759, P=0.029). No relationships were showed between HSPgp96 expression and sex,age and tumor size of colorectal cancer(P>0.05). (2) Expression of c‐jun The expression of c‐jun in colorectal cancer was higher than that in paracancer tissue(χ~2 =6.220, P=0.013). However, there was no statistic significance between c‐jun expression and other clinicopathlogic parameters. (3) Expression of CEA Positive expression of CEA in colorectal cancer were obviously higher than that in paracancer tissue(χ~2 =53.917, P<0.001); The expression rate in positive lymph nodes were higher than that of negative lymph nodes(χ~2 =4.532, P=0.033); Positive HSP gp96 expression of B stage + C stage of Dukes classification were higher than that of A stage + C stage(χ~2 =7.236, P=0.007). No statistically significant differences were showed between CEA expression and sex,age,tumor size and differentiated type of colorectal cancer(P>0.05). (4) Expression correlation of HSP gp96 and c‐jun and CEA In the 19 well differentiated cancer cases, there were 11 cases that the expression of HSP gp96 and c‐jun was commonly positive, and while 5 cases that the expression of HSP gp96 and c‐jun was commonly negative, Classified variable correlation analysis was applied to indicate that there was significantly positive correlation between expression of HSP gp96 and c‐jun (χ~2 =8.146, P<0.05, r=0.548). In the 69 cases which HSP gp96 expression was positive, there were 58 cases which CEA expression was positive. Classified variable correlation analysis was applied to indicate that there was significantly positive correlation between expression of HSP gp96 and CEA (χ~2 =7.686, P<0.05, r=0.431). (5) Expression and prognosis of HSP gp96 and c‐jun and CEA In the HSP gp96 positive expression group, 1 year,3 year,5 year survival rate were respectively 81.0%,49.6%,31.0%; while in negative group, 1 year,3 year,5 year survival rate were respectively 100.0%,87.5%,75.0%, the difference was significant(χ~2 =4.818, P=0.028). In the c‐jun positive expression group, 1 year,3 year,5 year survival rate were respectively 80.3%,51.5%,24.2%; while in negative group, 1 year,3 year,5 year survival rate were respectively 88.0%,60.0%,43.1%, the difference was not significant(χ~2 =2.582, P=0.108). And in the CEA positive expression group, 1 year,3 year,5 year survival rate were respectively 81.2%,47.0%,33.3%; while in negative group, 1 year,3 year,5 year survival rate were respectively 93.8%,81.3%,68.8%, the difference was significant(χ~2 =6.416, P=0.011). Other factors affecting overall survival were tumor size,depth of invasion of colorectal cancer,lymph nodes metastasis. Conclusions (1) HSP gp96 and CEA participated in development and metastasis of colorectal cancer. And c-jun promoted colorectal carcinogenesis. (2) The expression of HSP gp96 and CEA could judge prognosis of patients with colorectal cancer. (3) HSP gp96 may be a new tumor marker of colorectal cancer. (4)HSP gp96 may play roles in colorectal cancer through interacting c-jun and CEA.