| Objective:To compare the influence of Intrathecal injection of NGF(the test group), Intrathecal injection of NS(the control group)and musclar injection of NGF on functional recovery and nerve cells after spinal cord injury(SCI).Methods:52 rats were divided into three groups randomty,test group(n=20), control group(n=16)and musclar injection of NGF group(n=16). After spinal cord contusive injury was made in all these rats at T10 using modified Allen's method,the same volume of NGF solution (about 1.5μg pure NGF)and NS were intrathecal injection into the damaged areas respectively by the equipment which can afford a minimal injection. At the 24th hour,7th day,14th day,21th day,28th day after SCI, BBB Rating Scale was used to observe the recovery of motor function. And at the 7th day,14th day,21th day,28th day after injury, immunofluorescence and histopathologic examinations was performed to study the number of the adult endogenous neural cells and pathological changes after SCI.Results:From 24 hour to 4 weeks after SCI, the BBB scores of test group was higher than control group and musclar injection of NGF group. From 21 day,the scores of the test group was significantly higher than the control group .Histologic observation showed that the morphology of spinal cord and neurons in test group was better than that in control group and musclar injection of NGF group. The mean number of endogenous neural stem cells(NSCs) was more than that in control group and musclar injection of NGF group,while astrocytes was less than that in the other two groups. Conclusion: Intrathecal injection of NGF could significantly promote the number of endogenous NSCs and make them differentiate into neurons, thereby contributing to the repair of the motor function after spinal cord injury.
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