Effects Of SiRNA VEGF Gene Silencing On The VEGF Protein Expression Level Of The Transplanted Tumor In Nude Mice From Renal Cancer

Objective: To investigate the mechanism of the development of VEGF-siRNA in renal cancer, the effect of siRNA VEGF gene silencing on the VEGF protein expression level in a xenograft model of renal cell carcinoma was studied. Thus the gene therapy of renal cancer would be improved based on this research. Methods: The VEGF-siRNA sequence was chemosynthesis and selected in vitro in the previous experiments. After that the VEGF-siRNA sequence was transferred to the ACHN cell (Group A), the ACHN cell with transfer plasmid (Group B), and the ACHN cell without transfection (Group C) through liposome respectively. The nude mice were raised in SPF environment, and were observed on development of the transplanted tumor. The tumor dimension (length L, width W, and the volume V=1/2LW~2) was recorded every 5 days, while the tumor growth curve was drawn at the same time to show the inhibitory effects of siRNA on the tumor growth. The VEGF protein expression in the tissue section of the transplanted tumor in mice was also tested through immunohisto -chemistry method, to analyze the silencing effects of siRNA on VEGF expression. Results: The transplanted rumor developed and grew slowly in Group A. The tumor volume and weight of Group A were significantly lower than Group B and C (p < 0.05). The VEGF protein expression of Group A was significantly inhibited as indicated by the immunohistochemistry test (p < 0.05), while the Group B and Group C showed no significant difference in neither tumor volume and weight or VEGF protein expression (p > 0.05). Conclusions: The VEGF plays a important role in the development and growth of renal cancer. The chemosynthesis VEGF-siRNA could show specific inhibition on the VEGF expression in a xenograft model of renal cell carcinoma.