Effect Of VEGF-B Gene Targeted Sirna On The Growth Of Nude Mice Subcutaneous Implanted Tumor Of Human Hepatoma Carcinoma Cell HepG2Cell

Objective: To expose the effect of VEGF-B gene targeted small interferingRNA(siRNA) on biological characteristics of liver cancer and to open up anew targeted for gene therapy of liver cancer. We transfected stably humanhepatoma carcinoma cell(HCC) line HepG2cells by anti-human VEGF-B ge-ne targeted siRNA vector positive plasmid and implanted the cells which hada low expression of VEGF-B gene in nude mice to form subcu-taneous tumor.Methods: Three groups of human HCC line HepG2cells were transientlytransfected by three kinds of VEGF-B gene targeted siRNA vector plasmidand stably screened by blasticidin, these cells expressed lowly VEGF-B gene.As a control group, the HepG2cells were transfected by plasmid of no-loadvector. Finally, we obtained four groups of HepG2cells as follow. Threegroups of VEGF-B gene targeted siRNA plasmid(called Bs1group, Bs2group,Bs3group respectively) and1group of negative control no-load plasmid(called Bs0group). The four groups of HepG2cells were amplified andinoculated into four groups nude mice (six for each group, a total of24) toconstruct subcutaneous xenograft tumor model of human HCC respectively.They were named Ps1group, Ps2group, Ps3group and Ps0group respectively.Then, we measured the subcutaneous tumor's size and weight of every groupnude mice to study effect of VEGF-B gene targed siRNA on proliferation ofthe HCC cells and extracted tumor mRNA, detected expression of VEGF-Bgene by reverse transcription-polymerase chain reaction(RT-PCR) to studyeffect of VEGF-B gene targeted siRNA on the expression of VEGF-B.Results:1There were tumor function in nude mice after the nude mice wereimplanted with Bs0group, Bs1group, Bs2group and Bs3group of HepG2 cells for1week, and for5weeks average tumor volume were (2.274±0.451)cm~3,(0.709±0.333)cm~3,(0.698±0.614)cm~3, and (0.649±0.430)cm~3respectively,average tumor weight were (1.451±0.287)g,(0.507±0.212)g,(0.633±0.498)gand(0.583±0.343)g respectively. The tumor volume and tumor weight werelower in Ps1group, Ps2group, and Ps3group than that in Ps0group,respectively(P <0.05). There was no distant metastasis in each group.2Expression of VEGF-B gene in Ps0group, Ps1group, Ps2group and Ps3group were1.496±0.399,0.640±0.316,0.672±0.364and0.803±0.438respec-tively. It was obvious that VEGF-B gene expression of every experimentgroup was significantly lower than that control group(P <0.05).Conclusions: We established successfully subcutaneous xenograft tumormodel of human HCC(HepG2cell) in nude mice. VEGF-B gene targetedsiRNA vector positive plasmid can inhibit proliferation capacity of humanHCC line HepG2cells inoculated in nude mice, and decrease the VEGF-Bgene expression in nude mice.