Study Of The Expression And Clinical Significance Of Twist, E-Cadherin And β-Catenin In Colorctal Cancer

The EliVision^TM Plus immunohistochemical technique was used todetect Twist, E-cadherin,β-catenin and hybridization in situ technique wasused to detect E-cadherin in colorectal cancer, colorectal adenoma andcolorectal paracancerous normal tissues.It aimed to investigate belowaspects:To investigate the expression of Twist, E-cadherin,β-catenin in normalcolorectal tissue, colorectal adenoma, colorectal cancer tissue and theirrelationships with clinical pathological features.To investigate the relationships among Twist, E-cadherin andβ-catenin in colorectal cancer.To investigate the expression of E-cadherin mRNA in normalcolorectal tissue, colorectal adenoma,colorectal cancer tissue and theirrelationships with clinical pathological features, as well as the consistencyof the expression of E-cadherin using immunohistochemisty andhybridization in situ.Sixty cases of colorectal cancer, 20 cases of colorectal adenoma and20 cases of paracancerous normal tissues were collected from thedepartment of Pathology in the 251st hospital of PLA ranging from Januaryto June in 2004.The expression of Twist, E-cadherin,β-catenin weredetected in three tissues using immunohistochemistry technique ofEliVision^TM Plus. The expression of E-cadherin were detected in threetissues by using hybridization in situ. And the relationships among the threemarkers and the age, gender, the size of tumor, the degree of differentiation,TNM stage, the depth of invasion,metastasis of lymph nodes was analyzed,also the relationships among the three markers was analyzed. The consistency of the expression of E-cadherin in three tissues by usingimmunohistochemisty and hybridization in situ was analyzed.The positive rate of Twist,E-cadherin,β-catenin in colorectal cancerwas 53.3%(34/60), 35%(21/60), 18.3%(11/60); the positive rate of Twist,E-cadherin,β-catenin in adenoma was 10%(2/20), 70%(14/20),65%(13/20); the positive rate of Twist,E-cadherin,β-catenin inparacancerous normal tissue was 0%(0/20), 100%(20/20), 100%(20/20).The positive rate of Twist in colorectal cancer was significantly higher thanthat in adenoma and paracancerous normal tissue(P<0.05); the positive rateof E-cadherin,β-catenin in colorectal cancer was significantly lower thanthat in adenoma and paracancerous normal tissue(P<0.05). The positiverate of E-cadherin andβ-catenin in adenoma tissue was lower than that inparacancerous normal tissue(P<0.05); the positive rate of Twist in adenomatissue was higher than that in paracancerous normal tissue(P>0.05); theoverexpression of Twist and the abnormal expression E-cadherin andβ-catenin correlate with the tumor differentiation, depth of invasion, lymphnode metastasis, the TNM stage and 5 year survival rate, while notcorrelated with age,gender and the size of tumor.The positive cases of Twist,E-cadherin,β-catenin in the 60 colorectalcancer cases were 34, 21, 11 respectively. Seven positive cases ofE-cadherin and 2 cases ofβ-catenin aberrant expression were found in 34positive cases of Twist. Negative correlation was found through Spearmantest between the expression of Twist and E-cadherin；also between theexpression of Twist andβ-catenin.Positive correlation was also foundthrough Spearman test between the expression of E-cadherin andβ-catenin.The positive rate of E-cadherin mRNA was 38.3% in 60 cases ofcolorectal cancer tissue, 21 positive cases of E-cadherin protein were foundin 23 positive cases of E-cadherin mRNA. The positive rate of E-cadherinmRNA was 75% (15/20)in 20 cases of colorectal adenoma tissue, 14positive cases of E-cadherin protein were found in 15 positive cases of E-cadherin mRNA; the positive rate of E-cadherin mRNA was 100% in 20cases of paracancerous normal tissue. The positive rate of E-cadherinmRNA in colorectal cancer was significantly lower than in adenoma andparacancerous normal tissue(P<0.05). The positive rate of E-cadherinmRNA in adenoma tissue was lower than in paracancerous normaltissue(P<0.05); the abnormal expression E-cadherin mRNA correlate withthe tumor differentiation, depth of invasion,lymph node metastasis, theTNM stage and 5 year survival rate,while not correlated with age, genderand the size of tumor.There was no significant relation between the overexpression of Twistand the development of colorectal adenoma. Twist mihgt not take part inthe development of colorectal adenoma.High consistency were found between immunohisto- chemisty andhybridization in situ in detecting E-cadherin.Twist, E-cadherin andβ-catenin may play a cooperative role in theonset of malignant progression of colorectal cancers, and correlated withepithelial-mesenchymal transition significantly, which were importantbiological markers in the infiltration and early metastasis of colorectalcancer. Co-detection of Twist, E-cadherin andβ-catenin might be importantto evaluate the metastasis and prognosis of colorectal carcinoma. The useof Twist could be as a new prognostic marker of malignancy,and a potentialtherapeutic target in colorectal cancer.