The Role Of Activation Of The Lectin Pathway Of Complement In Pathogenesy Of IGA Nephropathy

ObjectiveComplements possesses many kinds of biological function,are used to participate in defencing reaction of organism antimicrobial generally, another aspect pose impairment of immunopathogenesis.With complement investigation continues deeply,MAC was detected in constitution and urine of many kinds of kidney disease,even complement dependence of HMC hyperplasy was acquired.HMC was activated with Activation of Pathway of Complement by mesangial region IgA deposition, mechanism of inducing IgAN glomeruli impaired was more and more payed close attention to. Etio-investigation perposes to identify the mechanism of in IgA nephropathy, to approach relationship between deposition of MBL and MASP and clinical situation and patho-change of IgAN, further to approach resource of deposition of MBL,to do provid experiment A/W with clinically effective healing. Materials and methods1.Enzyme-Linked Immunosorbent Assay was performed to detect mining level of serumal mannose-binding lectin (MBL) obtained from 20 patients with IgAN and 20 controls as normal child.Immunohistologic staining was performed on renal specimens obtained from 53 patients with IgAN and 23 controls as non- IgAN by using antibodies against MBL, MBL-associated serine proteases -1(MASP-1), C3,C1q,IgA and C5b-9 to comprehend mechanism of action Activation of the lectin pathway of complement in IgA nephropathy.2.Patients of IgAN were characterized into MBL-positive cases and MBL-negative cases by glomerular co-deposition of MBL and MASP ,and were analyzed retrospectively different distinction of couch expressionition,to comprehend relationships between the lectin pathway of complement and different couch expressionition; deposition intension of MBL and pathology classification of 22 MBL-positive cases were finished to analyze to comprehend ampho-relationships.3.HMC was cultured in vitro with patients serum of IgA nephropathy ,all were random separated into 0 hour group,6 hour group,12 hour group and 24 hour group,and all supernatant fluid was obtained. Enzyme-Linked Immunosorbent Assay was performed to detect mining level of supernatant fluid mannose-binding lectin (MBL) obtained from cell supernatant fluid of culture in vitro. Methods of RT-PCR was performed to check out expression of HMC MBLmRNA of 24 hour group,to comprehend HMC is the mechanism of activation the lectin pathway of complement in IgA nephropathy.ResultsThe first part the mechanism of activation the lectin pathway of complement in IgA nephropathy1.Glomerular deposition of MBL was observed in 22 of 53cases(41.5%) with IgAN and showed a mesangial pattern. All MBL-positive cases, but MBL-negative cases showed glomerular co-deposition of MBL-associated serineproteases, C3 and C5b-9 . Patients of IgAN with glomerular MBL deposition,pathology classification was not correlation with intension of MBL deposition. Intension of MBL deposition was positive correlation with IgA in patients of IgAN.2. Patients of IgAN , serum level of MBL was not showed significant difference than unimpaired child.3.MBL-positive cases were significantly more proteinuria as MBL-negative cases,and significantly more attacking macroscopic hematuria.The second part HMC is the mechanism of activation the lectin pathway of complement in IgA nephropathy1.Cell supernatant fluid of four groups were to compare, the mining level of MBLis cut down from 0 hour to 12 hour,there is distinction in principle of statistics(p=0.003); the mining level of MBLis step-up, there is distinction in principle of statistics(p<0.001).2.HMC by serum of IgA nephropathy illness co-culturing the expression of HMC MBL mRNA can be checked out,Conclusions1. Activation of the mero- lectin pathway of complement is significant action in IgA nephropathy.2. MBL is expressed in cultured HMC in votro,the development of disease in IgA nephropathy can be aggravated by HMC with the activation the lectin pathway of complement.