Influence Of RNA Interference Targeting Golgi P115 Gene On Apotosis Of Human Gastric Cancer Cells

Objective To investigate the influence and molecular mechanism of RNA interference targeting P115 on the apoptosis of human gastric cancer cells.Methods Using Eukaryotic expression plasmid which include shP115 Gene fragments to transfect into gastric cancer cells BGC-823, the transfection efficiency was confirmed by Fluorescence Microscopy,the expression levels of P115 and MIF were determined by RT-PCR and Western bloting,the change in the amount of nuclei - P53 protein was detected by Western bloting. Flow cytometry and MTT was used to determine Cell apoptosis and viability.Result To compared with other two control groups ,in the shP115-1318 group P115,MIF protein and mRNA expression were obversely reduced,the amount of nuclei - P53 protein was obviously added (P<0.05);FCM showed that the number of apoptosis cells in P115shRNA group was significantly more than the control groups(P<0.05);The results of MTT reviewed the activity of transfected group was much lower than control groups.Compared with the control group, the G1 /G2 phase proportion of the shP115 group at 48h was significantly higher, and the S phase proportion was obvious lower(P<0.05).Conclusion The Golgi-associated protein P115 play an adjustment role in cell apoptosis,and its mechanism is probably performed by regulating MIF factor.