| Objective:This study uses the rat model of cerebral ischemia and reperfusion injury to evaluate the neuroprotective efficacy of Danhong Injection and its mechanisms through the dynamic observation of morphological changes in brain nerve cells as well as Golgi and TGF-β1, GM130 expression changes.Methods:We build the cerebral ischemia reperfusion rat model through the middle cerebral artery occlusion (MCAO) method.112 healthy male SD rats were randomly divided into four major groups:normal group (N), sham operation group (S), ischemia-reperfusion injury group (I/R), the intervention group Danhong Injection (DI/R). Ischemic reperfusion injury group and the intervention group were sub-divided into cerebral infarction group and the immunohistochemistry group. Danhong in the intervention group was injected intraperitoneally 1 day before the experiment (8ml/kg, QD);ischemia-reperfusion injury group with the same dose of normal saline. Ischemia-reperfusion injury group and the intervention group were Danhong injected 2 hours after ischemia reperfusion. The animals were killed and samples of brain tissue were collected immediately at different time points:6 hours after reperfusion (6h),24 hours (24h),48 hours (48h),72 hours (72h), and 7 days (7d). The neurological deficit score, infarct size differences were compared in ischemia-reperfusion injury group and the intervention group by HE staining of nerve cells in the hippocampus CA1 number and immunohistochemistry of TGF-(31 in rat brain, as well as GM130 expression and morphological changes of Golgi apparatus. Data were analyzed with SPSS 18.0.Results:1. In addition to 6 hours of ischemia and reperfusion at different time points outside, intervention group Danhong Injection neurological deficit score was significantly better than the same time point of ischemia-reperfusion injury, and intervention in 7 days Danhong Injection group of neurological deficit score was significantly better than the rest of the time the intervention group (P<0.05)2. Danhong injection volume of cerebral infarction in rats intervention group was significantly less than ischemia reperfusion group (P<0.05)3. After ischemia reperfusion injury, the number of nerve cells in all the ischemic reperfusion injury groups reduced, and necrosis increased especially in 7 days. Danhong injection intervention group of neurons in the pathological damage was lighter than the same time point of ischemia-reperfusion injury, and 7 days Danhong Injection intervention group of neurons in the pathological damage was lighter than the rest of the time points in the intervention group.4. In the normal group, sham operation group, and Danhong Injection intervention group no significant change in Golgi morphology was observed at all time points i.e.6 hours,24 hours,48 hours,72 hours; on the time point of 7 days, some neurons in the normal Golgi network structure was absent of light pollution in ischemia group, and particles decreased or disappeared, and some breakage was observed.5. Ischemia-reperfusion injury group over time, TGF-β1 expression increased gradually, in ischemia-reperfusion injury group expressed the highest level for 48 hours, then decreased on 7 days significantly. Danhong injection after the intervention increased the expression of TGF-β1,in which seven days Danhong Injection intervention group was significantly higher than the other intervention group at each time point (P<0.05)6. Ischemia-reperfusion injury group over time, GM130 expression showed a decreasing trend, in ischemia-reperfusion injury in 7 days group was the lowest. Danhong injection GM130 expression increased after the intervention, which intervention Danhong injection 7 days group was significantly higher than the other intervention group at each time point (P<0.05)Conclusion:1. Ischemia reperfusion increased the expression of TGF-β1, decreased GM130 expression, and damaged Golgi stable.2. Danhong injection can increase the expression of TGF-β1 and GM130, maintenan stability in the Golgi and thus play a neuroprotective role.
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