| ObjectiveHepatic ischemia-reperfusion injury occurring after portal occlusion is a common phenomenon in hepatic surgical procedures and leads to higher postoperative morbidity and mortality. Danhong injection is known to protect ischemia-reperfusion injury in various organs and tissues. The purpose of this study is to investigate the protective effects of Danhong injection preconditioning on liver function ,histopathology,and inflammatory factors assessed after hepatic ischemia-reperfusion injury.MethodsFifty-four male Sprague-Dawle rats were randomly divided into three groups(n=18,per group): Sham-operation group(Sham group),Ischemia-reperfusion group(IR group) , Danhong injection pretreatment group(DH group). In the pharmacologic intervention group Danhong injection was administered intraperitoneally to rats(3.2ml/kg for 3 days)that were subsequently exposed to the hepatic ischemia reperfusion injury;In the Sham group and IR group rats were given the equivalent normal saline instead. Hepatic ischemia reperfusion was induced by clamping the hepatic artery,portal vein,and bile duct using a vascular clamp for 20 minutes,followed by reperfusion for 4,12 and 24 hour. Blood samples were collected after reperfusion to assay the levels of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6). Rats were humanely killed to obtain liver tissue to study for histopathologic assessment through HE staining and intercellular adhesion molecule-1(ICAM-1) expression through immunohistochemical staining.Results1. Protective effect of Danhong injection pretreatment on liver function after hep- atic ischemia-reperfusion. At 4,12,24 hour after reperfusion,Serum ALT,AST levels were significantly higher in the IR group compared with those of the Sham group(P<0.05),and pathologic injury was more severe than the Sham group;In the DH group,Serum ALT,AST levels were markedly decreased by pretreatment with Danhong injection compared with those of the IR group(P<0.05),and the abnormal morphological changes of hepatocytes were diminished remarkably.2. Influence of Danhong injection pretreatment on serum cytokines levels after hep- atic ischemia-reperfusion.At 4,12,24hour after reperfusion,Serum TNF-αand IL-6 levels were significantly higher in the IR group compared with those of the Sham group(P<0.05). Serum TNF-αand IL-6 levels were markedly reduced by pretreatment with Danhong injection compared with those of the IR group(P<0.05).3. Influence of Danhong injection pretreatment on expression of ICAM-1 after hepatic ischemia-reperfusion.Immunohistochemical staining showed that:In the Sham group,ICAM-1 expression of liver tissue was negative;In the IR group,at 4,12,24 hour after reperfusion ICAM-1 expressions in liver tissue were significantly increased in sinusoidal endothelial cells and small arteries muscle,small vein endothelial cells of portal area,part of the liver parenchymal cells could also be found the expressions of ICAM-1(P<0.01);In the DH group,At three time points after reperfusions ICAM-1 expressions in sinusoidal endothelial cells and liver cells were significantly reduced compared with the Sham group(P<0.01).Conclusion1.Danhong Injection pretreatment could significantly reduce the levels of serum ALT,AST,ameliorated the hepatocellular damage and protected liver function.2.Danhong injection pretreatment significantly decreased the levels of serum TNF-α,IL-6,reduced liver injury following hepatic ischemia reperfusion.3.Pretreatment with Danhong injection could markedly inhibit the liver over- expression of ICAM-1 after hepatic ischemia-reperfusion,which reduced neutrophil adhesion and aggregation,significantly attenuated the hepatic ischemia reperfusion injury.
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