Experimental Study Of Mobilizating Endothelial Progenitor Cells By G-CSF Combination With Simvastatin

Objective:contrasting the effect of bone marrow endothelial progenitor cells which has been drived by G-CSF and Simvastatin, G-CSF or Simvastatin.viewing the amount of peripheral endothelial progenitor cells at 5 time points, Select optimizing mobilization methods and occasions. To provide some new ideas and simple method for EPCs(endothelial progenitor cells) transplantated and other artificial stent to vascularize rapidly, as well as provide a reference for the tissue-engineering skin to vascularize.Methods:To choose raw material of Simvastatin(LuNan clique,ShanDong province, production number:050502) and rhG-CSF (QiLu tragacanth limited company production number:20100213) that have been used in clinical, to mobilizate by the dosage in accordance with the literature. Selected healthy mouse of KunMing species 120 (to avoid the effects of estrogen on EPCs), randomly divided into 4 groups:A group (G-CSF and Simvastatin intraperitoneal injection); B group (G-CSF intraperitoneal injection);C group (Simvastatin intraperitoneal injection), D group (negative control), each group setup 5 time points:3d,5d,7d,10d,14d.At each time point,selecting 6 mouse randomly and taking blood from heart to detect CD34, Flk-1, CD133-positive labeled cells by Flow cytometry. The results were used x±s to express and analyzed by statistics.Results:There is no significant difference in saline control group at 5 time points (p>0.05). the group of A,B and C double positive fluorescence cells-percentage increase gradually after mobilizing 3d,5d,7d and achieve the peak at 7d..Each time point, comparing with group D, group A,group B and group C double positive fluorescence cells-percentage increasing obviously (p<0.05);comparing with group B and group C. group A double positive fluorescence cells-percentage increasing obviously (p<0.05); comparing with group C, group B double positive fluorescence cells-percentage increasing obviously (p<0.05). After mobilizing 7d, taking group A and group D bone marrow cells and smearing.then view immunohistochemisty results:comparing with group D, group A positive cellspercentageA increasing obviously (p<0.05)Conclusion:G-CSF association with Simvastatin,G-CSF or Simvastatin all can mobilize the EPCs of bone marrow.After mobilizing,the EPCs quantity of peripheral blood can achieve peak at 7d.But group A is better than group B or group C, group B is better than group C.