PCR Detection Of Cytomegalovirus And Herpesviruses 6, 7, And 8 In Saliva From Human Immunodeficiency Virus-Infected Individuals In Yunnan, China

[Objective] AIDS is the human immunodeficiency virus (HIV) was caused by a global epidemic of acquired immune deficiency syndrome, and spread rapidly, high mortality, a serious threat to human life and health. Opportunistic infection is HIV infection hospitalization and mortality. Human herpesvirus (HHV) of such patients is common oral opportunistic pathogens, a serious threat of infection in patients with oral health and quality of life, is considered a common complication of infection. However, HIV infection of domestic oral herpes simplex virus type 5-8 (CMV, HHV-6, HHV-7 and HHV-8) infection in the whole of the situation, and the immune status and highly active antiretroviral therapy (HAART) between the study has not yet reported. The purpose of this project is the initial understanding of HIV patients in Yunnan oral herpes virus type 5 to 8 that CMV, HHV-6, HHV-7 and HHV-8 in the clinical characteristics and distribution in the saliva, research on HIV patients Early diagnosis and prevention-related oral lesions has important theoretical and practical significance.[Methods] (1) to collect between 2008-2010 to the Third People's Hospital, Kunming, treatment of a subject infected with HIV-infected people and 245 Yan'an Hospital of Kunming,30 healthy volunteers unstimulated whole saliva, while for the two groups The oral examination conditions and HIV-infected persons CD4 lymphocyte counts and other relevant informations. (2) the use of nested PCR * This study was supported by grants from National Natural Science Foundation of China (grant No.30860315) and Yunnan Province Natural Science Foundation (grant No.2008ZC146M) amplification methods such as human subjects saliva CMV, HHV-6, HHV-7 and the presence of HHV-8 DNA were detected, and a database using SPSS 18.0 software and associated indicators statistical analysis.[Results] (1) The demographic analysis indicated that participants of this study, male:female was 1.99:1 in HIV-infected patients,1.73:1 in control group. the majority(95.1%) of the patients being in 20 to 60 years, the average age of HIV-seropositive individuals was 34.45±10.78 years-old and 33.87±10.47 years-old for control subjects. the majority of the transmission was through injection drug use (43.7%),followed by sexual transmission with 39.6%; mode of transmission through transfusion was 0.4%; and unknown transmission was 16.3%. (2) Prevalence of CMV 34.7%, HHV-6 83.3%, HHV-7 70.2% and HHV-8 14.3% of saliva in HIV-infected patients, the corresponding control group were 10.0%,56.7%,70.0% and 0%. The overall detection rate of the two groups have differences (P<0.01). HIV group with or without oral mucosa lesions had no difference in the performance of the two groups (P> 0.05). There was no difference of detection rates of these four HHVs in saliva between HIV patients with HAART group and non-HAART group (P>0.05), detection rate of the four HHVs in saliva was not related with CD4 level (P>0.05).HAART group, non-HAART group compared with the normal group differences, and the three groups are present CMV, HHV-6, HHV-7 and HHV-8 multiple infections. Oral lesions and immune status of the main relationship, CD4 count was higher than that of non-HAART group HAART; major oral lesions in oral ulcers (RAU) in patients with CD4 counts higher than that of other oral lesions, Kaposi sarcoma of the lowest CD4 counts, body immunity to the worst. Epidemiological characteristics of the population a sense of HHVs were risk factors affect the detection rate of single, multi-factor analysis, gender, age, transmission route, CD4, CD8, packet type, oral lesions of other different effects of its existence.[Conclusions] HHV type 5 to 8 in saliva Detected by nested PCR analysis showed that oral herpes virus infection in patients with HIV infection is very common, of which the most prominent HHV-6 and HHV-7, HHV-8 minimum. HIV patients after HAART, the CD4 counts increased although their immunity improved ability to increase resistance to opportunistic infections, but oral herpes virus in the presence of the body in patients with no substantial impact, before and after treatment did not change its large, and there is a mixture of multiple viral infections. This study represents the first report of the prevalence of CMV,HHV-6,HHV-7 and HHV-8 in HIV-seropositive patients and HIV-seronegative individuals from mainland China and it has contributed baseline data and provided insights in viral OI and HIV co-infection in China. This would undoubtedly serve as a basis for further studies on this topic.