| Objective:Observing the influence of cyclophosphamide and busulfan on spermatogenesis in rats by different doses and treatments to build the best rat NOA model.Methods:160 adult Wistar rats were randomly divided into 10 groups(N=16). Group 1-3 were given busulfan 20mg.kg-1,15mg.kg-1,10mg.kg-1 by single intraperitoneal injection respectively. Group 4-6 were given cyclophosphamide 200mg.kg-1, 100mg.kg-1,75mg.kg-1 by single intraperitoneal injection respectively, Group 7-9 were given busulfan 40mg.kg-1,20mg.kg-1, CTX 200mg.kg-1 by single oral administration respectively, Groups 10 was given normal saline 2.0ml by single intraperitoneal injection. That the sperms and the sperm cells could not be found in the biopsy rat testis and epididymis after a reproductive cycle of 38 days probably is successful for building the NOA model. We can find the best way to build the model by comparing the number of spermatogenic cells and the weight of body, one side testis and epidiymis in successful group, lose group and placebo group.Results:38 days after our treatments, there were 2 rats,8 rats,15 rats,3 rats,10 rats, 15 rats,1 rats.2 rats,2 rats,16 rats survived in group 1-10, respectively.38 days after the treatments, the tissue biopsy of testis and epididymis showed the NOA model were successfully build in group 1,2,7 and 8. We assumed that the best way to build NOA model is giving Busulfan 15mg/kg by single intraperitoneal, which had the lowest mortality.Conclusion:NOA model was successfully built in Wistar rats. The way is giving Busulfan 15mg/kg by single intraperitoneal and it could resulted in the lowest mortality in rats. Objective:To assessment the influence of FSH and SCF to the spermatogenic function about rat models of NOA.Methods:The 72 rat models of NOA were prepared by injecting the busulfan which the dose is 15mg/kg each rat into the abdominal cavity.The models were divided into two groups after the success of preparing models:FSH and SCF were respectively injected into the hind leg muscle of each rat model twice a week with the five times doses to the total blood concentration of normal rat which were the treatment group. Equal amount of saline solution was injected as the same way which was the control group.The rat testis and epididymis of one side were cut down and were made biopsy on the 19th day,38th day and 57th after administration. Calculated the count of Spermatogonium,spermatocytes, sperm cells,sperm and the sperm in Epididymis tube on the vertical slice of seminiferous tubule and observed 25 compliant seminiferous tubules on each slice.Compared the slice results of different administration about the treatment group and control group by T test. and seted significant difference when p<0.01.Results:1:The permatogonium count of the treatment group and control group were respectively 58.00±15.70 and 30.90±13.52 after administrating 19 days,p=0.000;that about Spermatocytes were respectively47.44±15.52 and 12.65±8.08,P=0.000,that about sperm cells and sperm were respectively 12.42±8.11and 0,p=0.005.The count of sperm in the Epididymis tube about treatment group and control group were respectively 0 and 0 after administrating 19 days.2:The permatogonium count of the treatment group and control group were respectively66.77±20.56 and40.65±15.89 after administrating 38 days,p=0.000;that about Spermatocytes were respectively 56.82±21.93 and 18.50±13.66,P=0.000.that about sperm cells and sperm were respectively61.17±25.86and27.65±9.62,p=0.002.The count of sperm in the Epididymis tube about treatment group and control group were respectively77.55±55.18and41.05±29.20 after administrating 38days.3:The permatogonium count of the treatment group and control group were respectively78.63±24.28 and 47.95±18.12 after administrating 57 days,p=0.O00;that about Spermatocytes were respectively68.53±21.54 and 29.60±15.13,P=0.000,that about sperm cells and sperm were respectively102.83=35.31 and 45.15±20.96,p=0.000.The count of sperm in the Epididymis tube about treatment group and control group were respectively 122.13±80.67 and 58.50±26.15 after administrating 57days.Conclusion:FSH and SCF could improve the spermatogenesis recovery of wistar rat NOA models by injecting muscle.
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