| Objective:To investigate the effect of baicalin on apoptosis and the expression of apoptotic regulatory protein survivin in kidney of type 2 experimental diabetic rats. So to ascertain the anti-apoptosis of baicalin in the diabetic rat kidney.Methods:Thirty healthy male Wistar rats were divided randomly into three groups:the normal control group (n=10),the diabetes group(n=10)and the baicalin group(n=10). The normal rats were fed normal rat chow. while the other two groups were given high-fat, high-sugar diet for six weeks, the diabetes group and the baicalin group received intraperitoneal injection of streptozotocin once in a dose of 30mg/kg.After one week, the diabetes mellitus rat model was established. At the same time,150 mg·kg-1·d-1 of baicalin was administrated to the baicalin groups by oral gavaging, and the same volume normal saline was given to the normal control and the diabetes group for 8 weeks. All rats were weighted, then killed, and the fasting blood glucose, triglyceride, cholesterol, high-density lipoprotein, low-density lipoprotein were determined. TdT-mediated dUTP nick end labeling was used for the determination of kidney cell apoptosis. Reverse transcription-polymerase chain reaction was used for the determination of mRNA for survivin of kidney. Immunohistochemistry was used for the determination of protein expression for survivin of kidney。Results:compared with the normal control group, fasting blood glucose, triglyceride, cholesterol, low-density lipoprotein, Absolute and relative weight of kidney, Urine Volume for 24h, BUN, Creatinine and Total protein,poptosis index were all significantly higher in the diabetes group and the baicalin group (P＜0.01), body weight, high-density lipoprotein,the mRNA and protein expression of survivin of kidney was significantly lower in the diabetes group and the baicalin group (P＜0.01). Compared with the diabetes group, triglyceride, cholesterol, low-density lipoprotein,Urine Volume for 24h, BUN, Creatinine and Total protein,apoptosis index were all significantly lower(P＜0.01), but in the baicalin group, high-density lipoprotein, the protein and the mRNA expression of survivin of kidney was significantly higher(P＜0.05) in the baicalin group. body weight, fasting blood glucose, absolute and relative weight of kidney were insignificantly different from the diabetes group(P>0.05) compared to the DM group.Conclusion:Baicalin can reduce the apoptosis of renal cell in diabetic rats,the mechanism might be baicalin can up-regulate the expressions of inhibitor of apoptosis protein survivin in diabetic rat. Then improve the function of the system of anti -apoptosis in the kidney in the diabetic rats. |
PDF Full Text Request