| In this paper, zymotechnique, chemical compositions, anti-tumor and bacteriostasis in vitro of Naematoloma fasciculare fermented broth and fruit body were studied.On zymotechnique of N.fasciculare, obtain mycelium and secondary metabolites were studied by fluid submerged-culture method, determine the optimal carbon and nitrogen sources and inorganic salts by submerged fermentation culture, obtain Optimization medium compoundings by the response surface method, Optimization medium compoundings of N.fasciculare zymotechnique is maltose 59.27g/L, peptone:yeast powder=1:1 to 8.04g/L, potassium dihydrogen phosphate:Magnesium sulfate:sulfuric acid NH=1:1:1 was 2.27g/L, in a water bath oscillator at constant temperature, the speed is 120r/min, the temperature is 25℃, shaking 9d, biomass up to 15.06g/L. The culture medium is more reasonable than Yoshihito Shiono's, carbon, nitrogen and inorganic salts to match balance, and from the results of Box-Benhnken experimental analysis, the inorganic salt is more important on accumulation of N.fasciculare mycelia than nitrogen sources, inorganic salts are indispensable in the fermentation culture of Naematoloma fasciculare, and LD5o of Naematoloma fasciculare fermentation culture to mice was 3.15 g/kg。On chemical compositions, The chemical compositions of petroleum ether extract from N.fasciculare fermented broth and fruit body were similar by GC-MS analysis. N.fasciculare fruiting body was circumfluence extraction, separate chemical components to ethyl acetate extract by column chromatography, obtain five compounds, These compounds were identified by electron impact mass spectrometry or high resolution mass spectrometry,13C-NMR nuclear magnetic resonance spectroscopy,1H-NMR nuclear magnetic resonance spectroscopy, DEPT-90 and DEPT-135 NMR spectra. Compounds 1-5 were 1,3-diketone-4-dimethyl-10,13,14-trimethyl-15-hydroxy-17-(1-methyl-2,3-heptene acid)-8,9,11,12-diene tetro annulation triterpene,2,3-dioxy hydroxy-4-dimethyl-10,13,14-trimethyl-17-(6-hydroxy-4,5-heptene acid)-8,9,11,12-diene tetro annulation triterpene,4',5-dioxy-7-methoxy flavonone (sakuranetin),4,6,8(14),22(2,3)-tetraen-3-ketone ergostane and fasciculol B.On anti-tumor activity, taking H22 tumor-bearing mice, the doses of fermented broth were 200mg/kg, 400mg/kg,600mg/kg,800mg/kg,1000mg/kg, antitumor activity evaluate of N.fasciculare fermented broth by calculating the tumor inhibition rate, spleen index, liver index, thymus index, white blood cell count and IL-2 levels were measured, and compared with the control group of saline normal and the CTX group. According to the datas, we can see, the inhibition rate to the H22 tumor-bearing mice was 54.71% when the dose of N.fasciculare fermented broth was 200mg/kg, and the spleen index, thymus index, liver index, leucocyte count and IL-2 compared with the control group had improved in different extent, compared with the control group spleen index and the content of IL-2 showed significant difference (P<0.05), compared with the CTX group thymus and liver index group showed significantly different (P<0.05), compared with the control group the leucocyte count showed significantly different (P<0.01), compared with the CTX group the leucocyte count showed significantly different (P<0.05). The inhibitory rate was 30.38%,26.44%,10.65% when the doses of N.fasciculare fermentation broth were 400mg/kg,600mg/kg,800mg/kg, and compared with the control group the spleen index, thymus index, liver index, leucocyte count and IL-2 content decreased in different extent. The inhibition rate to the H22 tumor-bearing mice was -72.17% when the dose of N.fasciculare fermented broth was 1000mg/kg, and the spleen index, thymus index, liver index, leucocyte count and IL-2 levels were lower than Control group. There was on inhibition effect to SMMC-7721 of fasciculol B.On bacteriostasis in vitro, antimicrobial activity of in vitro to N.fasciculare fruiting body extracts obtained by refluxing extract, was run using scrip diffusion method, from the statistics of experimental datas, we can see, the inhibition rate to Bacillus coli ATCC8099 was 36.59% when the density of N.fasciculare ethyl acetate extract is 37mg/ml, compared with the positive drug group was very significantly inhibited (p<0.01). The inhibition rate to Staphylococcus aureus ATCC6538 was 84.38% when the density of N.fasciculare petroleum ether extract is 37mg/ml, compared with the positive drug group was very significantly inhibited (p<0.01). The inhibition rate to Bacillus subtilis ACCC11060 was 39.36% when the density of N.fasciculare chloroform extract is 37 mg/ml, compared with the positive drug group was very significantly inhibited (p<0.01). The inhibition rate to Agrobacterium tumefaciens EHA105 was 35.38% when the density of N.fasciculare petroleum ether extract was 18.5 mg/ml, compared with the positive drug group was very significantly inhibited (p<0.01). The inhibition rate to Staphylococcus aureus ATCC6538 was 12.27% when the density of compounds fasciculol B was 3×10-2mg/mL, The inhibition rate to Staphylococcus aureus ATCC6538 was 0.58% when the density of compounds fasciculol B was 6×10-2mg/mL. The inhibition rate to Staphylococcus aureus ATCC6538 was 0.19% when the density of 2,3-dioxy hydroxy-4-dimethyl-10,13,14-trimethyl-17-(6-hydroxy-4,5-heptene acid)-tetro annulation triterpene was 3×10-2mg/mL. The inhibition rate to Staphylococcus aureus ATCC6538 was 25.61% when the density of 1,3-diketone-4-dimethyl-10,13,14-trimethyl-15-hydroxy-17-(1-methyl-2,3-heptene acid)-8,9,11,12-diene tetro-annulation triterpene was 3×10-2 mg/mL. The inhibition rate to Agrobacterium tumefaciens EHA105 was 32.98% when the density of 1,3-diketone-4-dimethyl-10,13,14-trimethyl-15-hydroxy-17-(1-methyl-2,3-heptene acid)-8,9,11,12-diene tetro annulation triterpene was3×10-2mg/mL. The inhibition rate to Staphylococcus aureus ATCC6538 was 14.90% when the density of 1,3-diketone-4-dimethyl-10,13,14-trimethyl-15-hydroxy-17-(1-methyl-2,3-heptene acid)-8,9,11,12-diene tetro annulation triterpene was 6×10-2mg/mL. The inhibition rate to Agrobacterium tumefaciens EHA105 was 12.96% when the density of 1,3-diketone-4-dimethyl-10,13,14-trimethyl-15-hydroxy-17-(1-methyl-2,3-heptene acid)-8,9,11,12-diene tetro annulationtriterpene was 6×10-2mg/mL. There were no inhibition effect to Candida albicans JLC31680 and Candida albicans JLC31681 of N.fasciculare.
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