Study On Chemical Compositions And Pharmacal Activity Of Omphalia Lapidescens Schroet

In this paper, chemical compositions, anti-oxidation and bacteriostasis activity in vitro of Omphalia lapidescens were studied.On chemical compositions, Omphalia lapidescens sclerotium crushed was circumfluence extraction,six compounds were separated from the petroleum ether extraction of the sclerotium of Omphalia lapidescens by thin-layer chromatography and silica-gel column chromatography and identified by13C-NMR1H-NMR and MS. The six compounds are benzene butyl formate,4,6,8(14),22(23)-tetraen-3-one-ergostane, Ergosta-7,22,dien-3β-ol, Ergosta-5,7,22-triene-3β-ol, ergosterol and ergosterol peroxide.The benzene butyl formate,4,6,8(14),22(23)-tetraen-3-one-ergo-stane, Ergosta-7,22,dien-3β-ol, Ergosta-5,7,22-triene-3β-ol was separated from the sclerotium of Omphalia lapidescens for the first time. The chemical compositions of petroleum ether extract from Omphalia lapidescens was analyzed by GC-MS.On the antioxidation experiment in vitro,1,1-diphenyl-2-picrylhydrazyl radical (·DPPH), and hydroxyl radical (-OH) test systems were established to study the free radical scavenging activities of extractions of the sclerotium of Omphalia lapidescens.The experiment results show that acetic ether extraction has the best scavenging effect.-DPPH radical and·OH radical scavenging ratio of acetic ether extraction was97.15%and98.69%at2mg/mL.The second is methanolic extract, it’s·DPPH radical and·OH radical scavenging ratio is96.15%and77.83%at2mg/mL.The third is chloroform extract, it’s·DPPH radical and·OH radical scavenging ratio is67.61%and62.86%.The last is petroleum ether extraction, it’s DPPH radical and·OH radical scavenging ratio is20.07%and21.24%.On bacteriostasis in vitro, antimicrobial activity of in vitro to Omphalia lapidescens sclerotium extracts obtained by refluxing extract, was run using scrip diffusion method, from the statistics of experimental datas, we can see, all Omphalia lapidescens sclerotium extracts have different bacteriostasis effects. On anti-E. coli ATCC8099activity, EtOAc ether extract has best effect, the inhibition rate to E. coli ATCC8099was94.68%at50mg/mL.The second is Chloroform ether extract, the inhibition rate is76.78%at50mg/mL.The third is acetone and Petroleum ether extracts, the inhibition rate is61.72%and46.09%respectively at50mg/mL.The fourth is MeOH ether extract,, the inhibition rate is19.54%at50mg/mL.Water extract has no inhibitive effect to E. coli ATCC8099. In addition, the inhibition rate of compound2is the inhibition rate is16.36%at2×10-2mg/mL.Compound3and compound4have no inhibitive effect to E. coli ATCC8099.On anti-S. aureus ATCC6538activity, EtOAc ether extract has best effect, the inhibition rate to S. aureus ATCC6538was61.59%at50mg/mL.The second is acetone and chloroform ether extracts,the inhibition rate is52.27%and48.61%at50mg/mL. Petroleum ether and MeOH ether extracts has less effect, the inhibition rate is14.03%and20.38%respectively at50mg/mL.Water extract has no inhibitive effect to S. aureus ATCC6538. In addition, the inhibition rate of compound2is23.29%at2×10-2mg/mL.Compound3and compound4have no inhibitive effect to S. aureus ATCC6538.On anti-Bacillus subtilis ACCC11060activity, petroleum ether extract has best effect, the inhibition rate to Bacillus subtilis ACCC11060was56.09%at50mg/mL.The second is chloroform ether and EtOAc ether extracts,the inhibition rate is30.98%and20.54%respectively at50mg/mL. Scetone and meOH ether extracts has less effect, the inhibition rate is9.55%and8.07%respectively at50mg/mL. Water extract has no inhibitive effect to Bacillus subtilis ACCC11060. In addition, the inhibition rate of compound2is15.09%at2×10-2mg/mL.Compound3and compound4have no inhibitive effect to Bacillus subtilis ACCC11060.On anti-Blastomyces albicans JLC31680activity, EtOAc ether extract has best effect, the inhibition rate to Blastomyces albicans JLC31680was57.51%at50mg/mL.The second is chloroform ether and petroleum ether extracts,the inhibition rate is52.46%and30.55%respectively at50mg/mL. Scetone and meOH ether extracts has last effect, the inhibition rate is28.27%and15%respectively at50mg/mL.Water extract has no inhibitive effect to Blastomyces albicans JLC31680. In addition, the inhibition rate of compound2,Compound3and compound4have no inhibitive effect to Blastomyces albicans JLC31680.On anti-Blastomyces albicans JLC31681activity, EtOAc ether and Scetone extracts has similar effect, the inhibition rate to Blastomyces albicans JLC31681was21.39%and18.30%respectively at50mg/mL.Petroleum ether,chloroform ether,meOH ether and Water extracts have no inhibitive effect on Blastomyces albicans JLC31681. In addition, the inhibition rate of compound2,Compound3and compound4have no inhibitive effect to Blastomyces albicans JLC31681.