| Objective: Temporarily blocked by bilateral common carotid arteries in mice tail blood was prepared with an effective model of cerebral ischemia caused by VD. With the step down test and maze test of learning and memory in mice with VD and other cognitive functions, the use of serum enzyme assay of acetylcholine (Ach) content changes, observed by immunohistochemistry VD hippocampal tissue of mice Caspase-3 expression, as discussed Huazhuo detoxification, VD Huoxuetongluo Formula mice on the therapeutic effect and possible mechanisms.Methods: Mice with bilateral carotid artery occlusion recanalization open end of bleeding was prepared with an effective model of cerebral ischemia induced by VD. 45 male Kunming mice were randomly divided into 5 groups: model group, Huazhuo detoxification, blood Tongluo high dose group (hereinafter referred to as "high-dose group"), Huazhuo detoxification, Huoxuetongluo side low dose group (hereinafter referred to as "low dose"), nimodipine group (hereinafter referred to as "nimodipine"), sham operation group, n = 9. Four groups before bilateral carotid artery ligation method temporarily blocked blood flow, cerebral ischemia and reperfusion model of manufacturing the same time, 1cm away from the tip of the tail bleeding at about 0.3ml, thermal coagulation. Sham operation group were only isolated carotid artery, threading, but not ligation, and constantly tail, not bleeding. Successful model surgery 2 days after starting treatment were given, while the normal, equal feeding. Sham operation group, model group only with saline, nimodipine nimodipine group were fed liquid medicine high dose group, low dose group were given the high and low doses of Huazhuo detoxification Huoxuetongluo Formula fed , all day service once, and even served 15 days. 7,15 days after the first behavioral test, respectively (step down test, electric maze), the eye test in mice after the removal of blood, decapitation out mice brain tissue, by enzyme assay in serum Ach content changes, detected by immunohistochemistry in hippocampus of mouse brain expression of Caspase-3.Results:1 Huazhuo jiedu, huoxue tongluo recipe on VD model effects of cognitive function in mice1.1 Platform testAfter each stage, the model group than the sham response time was prolonged, the number of errors increased, the difference between the two groups was statistically significant (P<0.05). 7,15 days after the first test of the treatment group compared with the model group, reaction time and number of errors reduced. Reaction time of high-dose group was significantly shorter than the model group, the number of errors decreased, the difference was statistically significant (P<0.05). Results of the high dose group than the low dose group, the difference was significant (P<0.05).1.2 Electric maze test7,15 days after surgery were tested in the model group significantly increased the number of errors occurred, electric shock time was significantly higher than sham operation group, and the difference was statistically significant (P<0.05). The treatment group compared with model group, the number of correct judgments increased significantly shorter shock. Between the treatment group compared with the high dose group was significantly better than the low dose group, and statistically significant (P<0.05), high dose group and nimodipine group showed no significant difference between (P>0.05).2 Huazhuo jiedu, huoxue tongluo recipe on VD model in mouse serum of acetylcholinesterase (Ach) contentThe model group compared with the sham group, serum levels of Ach were significantly reduced, the difference was significant (P<0.01). Compared with model group, treatment group, Ach levels in serum were increased by the high dose group was significantly higher than the model group Ach, the difference was statistically significant (P<0.01); low dose group and model group no significant difference compared (P>0.05). Between the treatment group compared with the high dose group were significantly higher than that of Ach low dose group, the difference was statistically significant (P<0.05); high dose group and the nimodipine group than among the Ach content was no statistical difference significance (P>0.05).3 Huazhuo jiedu, huoxue tongluo recipe on VD models influence expression of Caspase-3 in hippocampus in brain tissue of miceModel group, low dose group in the VD of hippocampal tissue of mice at different time points were found in a large number of Caspase-3 activity in cells, and the model group and low dose was no significant difference between groups (P>0.05). Caspase-3 activity in which cell membrane integrity, and takes the shape of oval or spindle cell cytoplasm was stained brown by the cell body out of a few short processes, no significant branches. High dose group only shows a small amount of Caspase-3 activity in cells, significantly lower than model group and low dose group, and the difference was statistically significant (P<0.05), high dose group and nimodipine group Caspase-3 activity in hippocampus cells the number of the difference was not statistically significant (P>0.05).Conclusion:1 Huazhuo jiedu, huoxue tongluo prescription can increase VD mice experiment platform and maze of experimental results, improving their cognitive function.2 Huazhuo jiedu, huoxue tongluo prescription can rise VD model in mouse serum content of Ach, with improving learning and memory functions.3 Huazhuo jiedu, promoting blood circulation to remove obstruction party suppresses expression of Caspase-3 in hippocampus in brain tissue of mice, reduced VD apoptosis of cerebral neurons in mice.4 Improve Ach contents in serum of mice, inhibition of expression of Caspase-3 in hippocampus in the brain may be dark, detoxification and huoxue tongluo prescription on the treatment of one of the mechanisms of VD model mice. |
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