Expression And Significance Of CD147, MMP2 In Cervical Lesions

Objective: To investigate the expression of MMP2 and CD147 protein in CIN I-III and cervical squamous cell carcinoma and to clarify the possible role of extracellular matric (ECM) in the development or progression of cervical lesions. Then look for molecular markers of cervical cancer and precancerous lesions, and analyzed for the diagnosis, treatment, and predict the meaning of progress in cervical lesions.Methods: Twenty-one samples of normal cervix, 64 samples of CIN I-III (21 cases of CIN I, 22 cases of CIN II, and 21 cases of CIN III) and 35 samples of invasive carcinoma were collected. The expression of MMP2 and CD147 was examined by immunohistochemical assay. The expression of MMP2 and CD147 in CIN I-III, invasive carcinoma was compared with in cervicitis, respectively.Results: (1) The positive rates of CD147 expression were 5%(1/21), 95%(20/21), 91%(19/22),100%(21/21), 100%(35/35) in normal cervix, CIN I, CIN II, CIN III, and invasive carcinoma respectively. There were significantly differences in CIN I-III, cervical cancer and normal cervix (P<0.005). Compared to normal cervix, there were significantly differences in CIN I, CIN II, CIN III, and invasive carcinoma respectively (P<0.005). However, there were no significantly difference in CIN I-III and cervical cancer (P>0.05). The intensity of CD147 expression were significantly different in the above groups (x2=22.42, P<0.001). Pairwise comparison, there were no significant difference between CIN II and CIN III, or CIN III and invasive cervical cancer, the other groups were statistically significant. (2) The positive rates of MMP2 expression were 0(0/21), 71%(15/21),77%(17/22), 95% (20/21), 100%(35/35) in normal cervix, CIN I, CIN II, CIN III, and invasive carcinoma respectively. There were significantly differences in CIN I-III, cervical cancer and normal cervix (P<0.005). Compared to normal cervix, there were significantly differences in CIN I, CIN II, CIN III, and invasive carcinoma respectively (P<0.005). Pairwise comparison, there was significant difference between CIN I and invasive cervical cancer, the other groups were no statistically significant. The intensity of MMP2 expression were significantly different in the above groups (x2=21.57, P<0.001). (3) There was positive correlation between the expression of CD147 and MMP2 in cervical lesions (r=0.594,P<0.001).Conclusion: The expressive intensity and positive rates of CD147, MMP2 have correlation in the degree of cervical lesion. The expression of CD147 and MMP2 were positive correlation in the different cervical lesion. MMP-2 and CD147 may be meaningful molecular markers. The expression of MMP-2 and CD147 are related to the occurrence, development and progression of cervical lesions, and be used as new indicators to assess the severity of cervical lesion.