| ObjectiveBuilding renal VX2 cancer model by VX2 tissue mass planting method with small incision of the back, Evaluating the safety and reliability of this method which we constructed renal VX2 cancer model .The rabbit with renal VX2 cancer were treated by radiofrequency ablation.After this operation ,checking its peripheral blood cytokines .This paper discusses the changes in the peripheral blood cytokines of rabbit with kidney VX2 cancer,after radiofrequency ablation.Exploring the radiofrequency ablation causing changes of immune system and mechanism , when kidney VX2 tumors were treated by radiofrequency ablation.Methods20 New Zealand white rabbits will be constructed VX2 kidney cancer model by VX2 tissue mass planting method with small incision of the back.After 2 weeks ,the 20 New Zealand white rabbits will be sacrificed, to do the experiment autopsy and pathology examination, get kidney model building success rate and observe whether they have adjacent organs transfer and lymph node metastasis. 30 New Zealand big white rabbits were randomly divided into three groups, respectively, radiofrequency ablation group, postoperative therapy and the tumor control groups. After VX2 kidney model constructed,they were namely given radiofrequency, Radical nephrectomy and control treatments . After 7 days, days 14 and 28 days,the three groups were monitored the IFN-γ,TNF-αand IL- 2, 6, 10 content changes in the peripheral blood. 28 days after Processing,every experimental animals were executed pathology examination and CT imaging examination, compare postoperative CT imaging changes. Meanwhile every group of experimental animals were random selected two rabbits ,then were executed,observing postoperative pathology changes,on postoperative 7 days, and 14 days.After 28 days, the remaining rabbits were executed, and done pathology examination, the evaluating the effectiveness of radiofrequency ablation therapy.Results20 New Zealand big white rabbits VX2 renal cancer models were constructed successfully, All tumors are sporadic,The tumors were more exophytic sporadic and appearance rules .We did not see the renal capsule tumor invasion, relatively matching clinical early kidney features. Tumor-burdened rabbits grows stability;Radiofrequency ablation group of 10 rabbits were not found residual tumor tissue in pathological examination.The CT scans showed completely damaged tumor tissue.Radiofrequency treatment group compared with surgical treatment group Postoperative 7 days, IFN-γconcentration increases, and IL - 2, IL - 6, TNF–αand IL - 10 concentration were no obvious difference; Postoperative 14 days comparison: IL - 6 concentration was reduced, and the elevated IFN–γconcentration, IL - 2, TNF–αand IL - 10 concentration no obvious difference; Postoperative 28 days comparison: IL-2 and IFN–γincreases, IL-6 and IL-10 concentration lower,that TNF–αconcentration was no obvious difference.Radiofrequency treatment group compared with tumor control group, Postoperative 7 days comparison: IL-6 and TNF-αwas reduced,IFN–γwas increased, IL-2 and IL-10 concentration were no difference; Postoperative 14 days comparison: IL - 2 and IFN–γrose, IL- 6, and IL-10 concentration were lower; Postoperative 28 days comparison: IL-2 and IFN-γconcentration increased, IL- 6 and IL-10 concentration decreased ,that TNF-αconcentration were no obvious difference.Surgical treatment with tumor control group of seven days after comparison: IFN-γ, IL-10 and IL-2 concentration were no obvious difference, and TNF-αand IL - 6 decreased; 14 days comparison: IL - 6 and IL - 10 concentration decreased, IFN-γconcentration increased, IL - 2 and TNF-αconcentration were no obvious difference; Postoperative 28 days comparison: IL - 2 and IFN-γconcentration increased, and IL - 6 and IL - 10 concentration was lower ,that TNF-αconcentration was no difference.ConclusionThe method of costrcuting rabbit kidney VX2 cancer mode by VX2 tissue mass planting method with small incision of the back is very ideal .It is simple, It has a high success rate and is biology stable model.Radiofrequency ablation therapy can not only safely and effectively damages the tumor, but also cause immune system changes, and enhance the antitumor immune. It can cause IL– 2 and IFN-γcontent increased, IL - 6 and IL - 10 content low in the peripheral blood of experimental rabbit. Its mechanism may be the tumor tissue necrosis were fully damaged, immune inhibition were reduced, especially IL - 6 and IL-10 immunosuppression factor levels are low, It cause the immune system activation. At the same time because the damaged tumor tissue were left in body, tumor antigen releases, which causes the immune system activation. The cytokines in peripheral blood changes.The IFN -γ, IL-2 content increased, IL-6 and IL-10 content is reduced in the peripheral blood of radiofrequency ablation group, It cause strengthen function of CD4 + T cells, NK cell number and other lymphocy, organisms antineoplastic immune enhanced.
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