Effect Of Compound Radix Sophorae Flavescentis Injection On Hedgehog Signaling Pathway Activation In Rat Hepatocarcinoma Models

0bjectiveTo study the role of hedgehog signaling pathway activation in artificially induced-SD rat hepatocarcinoma and to investigate the effect of compound radix sophorae flavescentis injection (CRSFI) on hedgehog signaling pathway.MethodsSD rats were established diethylnitrosamine-induced hepatocarcinoma models, and then randomly divided into model group, low-dose CRSFI group and high-dose CRSFI group, at the same time, the control group was established. Rats in the low- and high-dose CRSFI groups were given the treatment of CRSFI concentration for 30 and 90 mg/kg/d, for 3 weeks by intraperitoneal injection at 15 weeks after modeling. The expression of target gene transcription factors Gli2 and transmembrane protein receptor complex Ptch in the hedgehog signaling pathway was detected by immunohistochemical staining and RT-PCR. Results1.SD rats hepatocarcinoma models were established by diethylnitrosamine-induced.2.The expression of Gli2 and Ptch was high expressed in the model, low- and high-dose CRSFI groups compared with the control group (P < 0.01).3.The expression of Gli2 and Ptch in Hedgehog signalling pathway is highly positive after treated by different dose CRSFIwith the model group (P < 0.01).4.the expression of Gli2 and Ptch in Hedgehog signalling pathway were negative or weakly observed by different dose CRSFI,it is non-statistical significance(P>0.05).Conclusion1.Rats hepatocellular carcinomas model were successfully induced by induced DEN. The procession of hepatocarcinogenesis included three stages:hepatic toxic lesion,hepatic proliferation/cirrhosis and hepatic carcinogenesis.the model is an ideal experimental animal model for the clinical medicine study of human hepatocarcinoma.2.The results suggest that the hedgehog signaling is abnormally activated in artificially induced-SD rat hepatocarcinoma. 3.In different dose CRSFI groups,the expression of Gli2 and Ptch was highly positive,The CRSFI probably inhibit the hedgehog signaling pathway.