An Experimental Study Of Radix Sophorae Flavescentis In Antagonizing Hypersensitivity Type Ⅰ

Objective:To study the effects and pharmacological mechanism of radixsophorae flavescentis (RSF) in treating experimental hypersensitivity type in vivo compared with TMZF.Methods:(1) Anti-serum with IgE of rats administrated with ovalbumin was prepared for the experiment first.(2) The passive cutaneous anaphylaxis (PCA) rat model of hypersensitivity type I were induced by intradermal injection of rat anti-ovalbumin serum, the rats with PCA were divided into six groups and administrated with the corresponding drugs:the normal group(NS), the control group(NS), the treatment group of RSF in two concentrations (1.4g/ml and2.8g/ml), the Tuo Min Zu Fang (TMZF,2.8g/ml) and the treatment group of Ketotifen (4mg/kg), then they were attacked by ovalbumin and Evans’blue. The effusion of Evans’blue from the skin being passive allergized were detected spectrophotometrically at610nm, and the inhibition ratio to PCA were used to show the effect of RSF to hypersensitivity type I.(3) The morphologic changes of mast cells in rat calvarial periosteum allergized by anti-ovalbumin serum were observed to study the effects of RSF in stabilizing the membranes of mast cell in vivo.(4) RSF could inhibited mast cells allergized by anti-ovalbumin serum to release histamine in vitro. The inhibiting effects were lower than those of TMZF and Ketotifen.Results The ratio of effusion of Evans’blue at the allergized skin and of the degranulation of mast cells in rat calvarial periosteum of rats in the treatment group (with1.4g/ml and2.8g/ml of RSF respectively) were lowered markedly as compared with that in the model group (p<0.01). The effect of RSF inhibiting anaphylactic reaction and histamine release were lower than that in TMZF group and Ketotifen in vivo and in vitro. Conclusions:It is suggested that RSF possesses an antagonistic effect on hypersensitivity type I by inhibiting the degranulation of mast cells and by blocking the combination of inflammatory mediators released from mast cells with target cells. However the role of RSF anti-hypersensitivity is much less than TMZF.