The Expression And Significance Of P57^kip2 And Cyclin D1 In Gastric Cardia Cancer And Precancerous Lesion

Objective Clinical significance and relation of the p57^kip2,cyclinD1 expression in gastric cardia cancer and different grade lesions of gastric cardia tissue.Methods One hundred and thirty case of gastroscope biopsies and surgical specimens of gastric cardia were collected at The first affiliated hospital of Henan university of science and technology. All specimens was divided into 4 groups: gastric cardia hilitis 24 case; gastric cardiac atypical hyperplasia 26 case;gastric cardia cancer 60 case and The control group: normal gastic cardia mucosa 20 case.The expression of p57^kip2,cyclinD1 were detected in every group by immunohistochemical S-P methods; The difference of the expression of p57^kip2,cyclinD1 were analyzed during every group by SPSS. Analyzed the correlation between gastric cardia cancer. The results of immunohistochemistry and clinicopathological parameters were analyzed.Result The expression of p57^kip2 in different gastric cardia tissue:the positive expression rate in gastric cardia hilitis group 87.5% (21/24) is significant higher than gastric cardia atypical hyperplasia group 53.8% (14/26) (P<0.05),there is no significant difference between gastric cardia atypical hyperplasia group and gastric cardia cancer group 43.4% (26/60) (P>0.05) ,with the advancing of disease,the positive expression rate decreased gradually. By the statistic analyzed,the expression of p57^kip2 were different among 4 groups.The expression rate of p57^kip2 in normal mucosa is 90% (18/20),and in gastric cardia cancer tissue is 43.4% (26/60),the different between them were obviously.The expression of p57^kip2 protin rate is 71.4% (10/14) in stage I gastric cardia cancer,41.4% (12/29) in stage II and 23.5% (4/17) in stage III,There are statistic significant in this results (P<0.05).The expression of p57^kip2 in well differerntiated and moderately differentiated 66.7% (8/12),57.1% (12/21) were significantly higher than poorly diffierentiated 22.2% (4/17) (P<0.05). The positive expression of p57^kip2in the group with lymph node metastasis is 44.1% (15/34) and not significant different than the group without lymph node metastasis 42.3% (11/26). The expression of cyclinD1 in different gastric cardia tissue:the positive expression rate in gastric cardia cancer group 53.3% (32/60) is significant higher than gastric cardiac atypical hyperplasia group 30.8% (8/26) (P<0.05),there is no significant difference between gastric cardia atypical hyperplasia group and gastric cardia hilitis group 29.2% (7/24) (P>0.05) ,with advancing of disease,the positive expression rate increased gradually. By the statistic analyzed,the expression of cyclinD1 is different among 4 groups. The expression rate of cyclinD1 in normal mucosa is 25% (5/20),and in gastric cardia cancer is 53.3% (32/60),the differences between them are obviously.The expression of cyclinD1 protin rate is 28.6% (4/14) in stage I gastric cardia cancer, 51.7% (15/29) in stage II and 76.5% (13/17) in stage III, There are statistic significant in this results (P<0.05).The expression of cyclinD1 in well differerntiated , moderately differentiated and poorly diffierentiated is 58.3% (7/12),52.4% (11/21) and 51.9% (14/27) respectively,there is no statistical sense among the three groups.The positive expression of cyclinD1 in the group with lymph node metastasis is 70.6% (24/34) and it is significant higher than the group without lymph node metastasis 30.8% (8/26). The relation between p57^kip2 and cyclinD1:There is 5 specimens expressed cyclinD1 (19.2%) in the 26 p57^kip2 positive gastric cardia cancer specimens,and 29 specimens expressed cyclinD1 (85.3%) in 34 p57^kip2 negative gastric cardia cancer specimens,suggest that p57^kip2 and cyclinD1 has negative correlation in gastric cardia cancer tissue (r=-0.661,P<0.01).Conclusion 1.The difference of the expression of p57^kip2 protin in gastric cardia hilitis; gastric cardiac atypical hyperplasia and gastric cardia cancer has statistic sense,and correlate wih gastric cardia cancer stages and differentiation grade.Suggest that loss of p57^kip2 gene expression has relation with the occurrence and progression of gastric cardia cancer.The expression level of p57^kip2 protin maybe a mark to determine the malignancy grade of gastric cardia cancer,and may guide the clinical diagnose. 2. The difference of the expression of cyclinD1 protin in gastric cardia hilitis; gastric cardiac atypical hyperplasia and gastric cardia cancer has statistical sense,and correlate wih gastric cardia cancer stages with or without lymph node metastasis.Suggest that the detection of cyclinD1 may helpful to the diagnose of gastric cardia precancerous lesion and earlier gastric cardia cancer and this protin may an important role in the progression in gastric cardia cancer. 3.Both p57^kip2 and cyclinD1 are importment cell cycle control genes, Loss of p57^kip2 expression and high cyclinD1 expression may have synergistic action in the occurrence of gastric cardia cancer, joint detection about p57^kip2 and cyclinD1 may provided more biological information for the clinical detection and diagnosis of gastric cardia cancer.