The Significant Of The Expression Of Ki-67/MIB-1, MMP-2 And TIMP-2 In Bladder Transitional Cell Carcinoma

Objective: To explore the expression of nuclear associated antigen (Ki-67/MIB-1) and matrix metalloproteinase (MMP-2) and its tissue inhibitor (TIMP-2) in transitional cell cancer of bladder (TCCB) and the relationship between pathologic factor of tumor, relapse and prognosis. Methods: Applying S-P immunohistochemical technique to detect the expression of Ki-67/ MIB-1, MMP-2 and TIMP-2 at 50 urine bladders cancer specimens ,and analyse clinical data. The yellow particles inside cell plasma under microscope were positive cell, count five sight continuously, Number of positive cell>25% was positive and <25% was negative. statistic treatment: all data were input computer and were treated with SPSS10.0stastic software。Calculated data were with X2 test or FISHER test and there was significantly difference when P<0.05. Results: 1. The expression of Ki-67/MIB-1, MMP-2 and TIMP-2s is negative in normal bladder tissue. 2. The positive expression rate of Ki-67/ MIB-1, MMP-2 and TIMP-2s is 52% (26/50), 48% (24/50) and 58% respectively. (29/50). The expression rate of Ki-67/ MIB-1, MMP-2 increased along with pathnological stage and grade while TIMP-2 expression rate tend to decrease. There was significantly difference between pathnological stage and grade of the expression of Ki-67/MIB-1, MMP-2, TIMP-2 (P<0.05). 3. In 45 case, the tumor relapse rate of Ki-67/MIB-1 positive expression is higher than negative expression, the relapsing rate is 54.2% (13/24) and 23.7% (5/21) 34.7% (8/23) respectuvely. the relapsing rate of TIMP-2 positive expression is 37% (10/27), and the relapseing rate of TIMP-2 negative expression is 44.4% (8/18). there was no significant difference between MMP-2 positive expression and MMP-2 negative expression patients. 4. In 45 case, ththe positive expression rate of MMP-2 in those cases whos died and survived is 75% (6/8) and 43.2% (16/37) respectively. there was significantly difference between them (P<0.05). the positive expression rate of TIMP-2 in those cases who died and survived is 50% (4/8) and 62.2% (23/37) respectively. Ther were no significantly difference between them (P>0.05). that indicated that the positive expression of Ki-67 (MIB-1) and MMP-2 is correlation with the prognosis of transitional cell cancer of bladder (TCCB). Conclusion 1. Expression rate of Ki-67 (MIB-1), MMP-2, TIMP-2 were closely correlated with pathological stage and tissue grade of TCCB. 2. the Ki-67 (MIB-1) expression rate were closely associated with recurrence of TCCB. 3. Ki-67 (MIB-1)and MMP-2 expression rates were closely associated with prognosis of TCCB.that indicated that Ki-67 (MIB-1) and MMP-2 expression rates will be a factor to judge the prognosis of TCCB. These data demonstrated that parts of transitional cell cancer of bladders were negatively express of TIMP-2 and positively express of MMP-2and Ki-67 (MIB-1)。Moreover, the TIMP-2 were closely negative related with the expression of two. others protein. That indicated TIMP-2 MMP-2 and Ki-67 (MIB-1)s played an important role in occurrence and development of transitional cell cancer of bladder, and may affect the process of occurrence and development of transitional cell cancer of bladder together. Its function mechanism needs further research still, and more data will be collected at the same time. Periodically visit is also a focus of next study to evaluate the prognosis of TCCB applied with this result.