Investigation Of SiRNA Targeted To Survivin Gene In Suppressing Proliferation And Inducing Apoptosis In Laryngeal Carcinoma

Objective:To investigate the effect of blocking the survivin gene expression with RNA interference (RNAi) techniques on suppressing proliferation and inducing apoptosis of laryngeal carcinoma Hep-2 cells,in order to supply an effective target and technique for laryngeal carcinoma gene therapy. Methods:A siRNA directed against survivin gene was transfected into laryngeal carcinoma Hep-2 cells with lipofectamineTM2000. The effect of suppressing proliferation of Hep-2 cells was detected by MTT[3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The changes of survivin mRNA expression were detected by semi-quantitive RT-PCR. The protein of survivin was evaluated by means of Western blot. The effect of inducing Hep-2 cells apoptosis was detected by flow cytometry. Results:The inhibition ratio of proliferation and the apoptotic ratio were significantly higher than those of control groups (P < 0. 05); Expressions of survivin gene in mRNA and protein levels were suppressed by survivin-siRNA in a dose-dependent manner. Conclusion:Blocking the survivin expression with RNAi technology can significantly suppress proliferation of Hep-2 cells and induce apoptosis to a certain degree. Expressions of survivin mRNA and protein in different dose siRNA groups were decreased in different degrees.RNAi targeted to survivin gene has a potential value in gene therapy of laryngeal carcinoma.