The Advantages Of A Novel CoQ10 Delivering System In Skin Aging Protection

Skin photo-ageing induced by ultraviolet(UV)radiation is mainly ascribed to oxidative stress and reactive oxygen species(ROS). Coenzyme Q10(CoQ10)has been reported as a powerful antioxidant in plasma. However, CoQ10 was barely satisfactory in topical drug delivery because of its lipid solubility. Nano-structured lipid carriers(NLC)are novel drug delivery systems with high substance solubility, stability, powerful skin penetration and low skin irritation.To improve the anti-oxidative efficiency of CoQ10 in skin photo-ageing, the present research prepared a novel CoQ10 nano-structured lipid carrier(CoQ10-NLC) and characterised it by size and morphology with photon correlation spectroscopy, freeze-fracture transmission electron microscopy(FF-TEM)and Cryo-TEM.We built a UVA-irradiated model with human embryo skin fibroblasts (ESF-1) to mimic the nature skin photo-ageing. In UVA-irradiated fibroblasts, the protection of CoQ10-NLC was more effective than the CoQ10-emulsion as demonstrated by cell viability and morphological changes of the cell body and nucleus. In addition, malondialdehyde ( MDA, the product of lipid peroxidation) concentration decreased by 61.5% in the group treated with CoQ10-NLC compared to the group subjected to general CoQ10 emulsion. In the presence of CoQ10-NLC, the activities of the anti-oxidative enzymes superoxide dismutase(SOD)and glutathione peroxidase(GSH-px)were reinstated to 81% and 75%, respectively, of the control group.To evaluate the penetration ability of CoQ10-NLC in vivo, we did topical skin penetrated experiment with rat skin. The CoQ10-NLC displayed a stronger capability to penetrate the stratum corneum and permeate the dermis after a topical skin application.These results reveal that CoQ10-NLC has greater antioxidant properties and topical skin penetration than the CoQ10-emulsion.