| Delayed neuropsychological sequelae after acute carbon monoxide poisoning ( DNS) is one of serious complications of acute carbon monoxide poisoning. DNS develops later following a symptom-free interval of a few weeks or longer. Once the disease occurs, there is no ideal clinical treatment method. The key is early diagnosis, active prevention. So far, we do not make the complicated mechanism of DNS clear.Research primarily considered that acute ischemia and hypoxia, cytotoxic injury, reperfusion injury and free radicals, excitatory amino acids and cell apoptosis, immune dysfunction and other related disorders of neurotransmitters might be involved after carbon monoxide poisoning. Magnetic resonance spectroscopy (MRS) is only an invasive method that can detect tissue metablism, biochemical circomustance and make quantitative analysis of compound in live body. It can collect the signal of compond in every interested area. It can reach the purpose for ration measure and analysis of metablism in human body by reflecting the function relation curve between signal intensity and resonance frequency. So, it can provide a new approach to study the changes of metabolite during DNS process.As a new generation of anti-oxidants, Edaravone can remove free radicals, inhibit lipid peroxidation, DNA oxidative damage and delayed neuronal death. So far, little is reported about edaravone in DNS prevention and treatment.Objective:To elucidate role of free radicals in DNS, we observed effect of edaravone on DNS rabbit serum superoxide dismutase (SOD) activity, malondialdehyde (MDA) levels, the level of relative value of MRS parameters (NAA, Cho, Lac, Cr, etc.) and neron numbers of happocampal CA1 area.Methods:Rabbits were randomly divided into normal group, DNS group, saline group, edaravone group. Rabbit DNS model was made by intraperitoneal injection of CO. Normal saline (1mg/Kg, twice daily, a total of one course of 14 days) was utilized through the ear vein in saline group, and edaravone was utilized through the ear vein (1mg/Kg, day 2 times, a total of one course of 14 days) in edaravone group. Serum superoxide dismutase (SOD) activity and malondialdehyde (MDA) was measured in each group. Measurement of various compounds the relative ratio of region of interest was applied in brain MRS examination. Neurons in hippocampal CA1 area was counted by microscopy. Results:Compared with normal group, there is significantly decreased serum SOD activity and increased MDA content in DNS group. The ratio of NAA / Cr significantly decreased in DNS group, while the ratio of Cho / Cr and Lac / Cr significantly increased in hippocampus region of temporal lobe (P <0. 01); Compared with normal group, there is no significant difference in serum SOD activity, MDA content, the ratio NAA / Cr, Cho / Cr and Lac / in the hippocampus region of temporal lobe Cr (P> 0.05 ). Compared with DNS group or saline group, serum SOD activity significantly increased and MDA levels significantly decreased in Edaravone group(P <0. 01), while the ratio of NAA / Cr was significantly higher, ratio of Cho / Cr and Lac / Cr significantly reduced in hippocampal region of temporal lobe (P <0. 01). Compared with normal group, there is significantly decreased neuron number of hippocampal CA1 area in DNS group (P <0. 01). Compared with saline group, neuron number of hippocampal CA1 area increase significantly in edaravone group (P <0. 01).Conclusion:Our results indicate that: 1) Free radicals are involved in the process of DNS;2) Brain MRS might be used for early diagonos and beneficial to estimate therapeutic effect of DNS;3) Edaravone can be used for the prevention and treatment in DNS.
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