Use SELDI-TOF-MS To Screen Serum Biomarkers With Early Onset Severe Preeclampsia And Establish The Serum Diagnostic Proteomic Pattern

Objective:we studied total protein of serum by using SELDI-TOF-MS technology in this study,by comparing the differences of protein expression in serum of ES-PE,LS-PE and normal pregnant women in hope of finding specific biomarkers of ES-PE,so as to elucidate the pathogenic mechanism of ES-PE.Method:We collected serum from severe preeclampsia patients and normal pregnant women.subgroups:â‘ early onset group:12 patients with early onset severe preeclampsia.â‘¡late onset group:19 patients with late onset severe preeclampsia.â‘¢normal group:19 normal pregnant women whose gestational week corresponded to early onset severe preeclampsia. The sera come from this fifty patients were tested by surface enhanced laser desorption/ionization-time of flight-mass spectrometry and got finger printings.The differential proteins among this three groups were analyzed by ProteinChip Software 3.2.1 BioMarker Wizard and several specific biomarkers of ES-PE were found.Then we used training cluster including 32 of 50 to set up diagnostic patterm of early onset severe preeclampsia with Biomark Pattern Software 5.0. Using this diagnostic patterm we gave a double-blind trial to the remanent serum samples from patients to get its sensitivity and specificity. At last we searched for the specific differential proteins of early onset severe preeclampsia we found on the Swiss-Prot and TrEMBL database according to their molecular weight.Result:1. In the serum of early onset group,the relative amount of proteins which M/Z are 7815,8348,4419,6926 is obviously lower than normal group, the relative amount of proteins which M/Z are 5802,4791,4438 is obviously higher than normal group.2. In the serum of early onset group,the relative amount of proteins which M/Z are 7815,8348,4057 is obviously lower than late onset group,the relative amount of proteins which M/Z are 5802,5629 is obviously higher than late onset severe preeclampsia group.3. The proteins which M/Z are 7815,8348,5802 are the special biomarkers in serum of early onset severe preeclampsia. We searched for this three specific proteins on the Swiss-Prot and TrEMBL database according their MW. Result:the protein which MW is 5802Da may be osteocalcin or c-type natriuretic peptide;the protein which MW is 8348Da may be platelet basic protein-like 2 or Small-inducible cytokine A14; the protein which MW is 7815Da may be platelet factor 4 variant 2, macrophage inflammatory protein 1-beta or epithelial-derived neutrophil-activating protein 78;4.The diagnostic patterm built by BPS consists of 4 peaks which M/Z is 5802,5629,2191,2632.The sensitivity of the diagnostic pattern to the all specimens is 91.7%,and the specificity is 94.7%.Conclusion:1. The serum differential proteins between early group and normal group may be the specific biomarkers which participate the development procedure of early onset preeclampsia and can diagnose it.2. Existence of differential proteins between early group and late group shows that the pathogeneses of early onset preeclampsia and late onset preeclampsia maybe different.3. The serum differential proteins which M/Z are 7815,8348,5802 may be the special biomarkers of early onset severe preeclampsia.The result of searching database shows that the development of early onset preeclampsia may correlate to platelet function,inflammatory reaction, calcium metabolism.4. The diagnostic patterm built in this study maybe useful for diagnosis of early onset preeclampsia.