| Panax quinquefollium L. is a famous and precious herb that belongs to the family Araliaceae. The quality of Panax quinquefolium L. is different with the varieties, origin, harvest season, medicinal parts, processing methods and storage conditions. Now, But for the undound quality evaluation standard system, it is still very difficult to identify the different parts of the body which possesses the same genetic type. This seriously influenced the development of Chinese quinquefolium L. industry. So one of the most important problems that need to be resloved is how to improve the internal quality evaluation criteria of quinquefolium L. with the modern biological technology. In this paper, two-dimensional electrophoresis (2-DE) system for which suitable quinquefolium L. proteomic analysis was first established, and then the form and expression differences of proteins in different parts of quinquefolium L. root were systematically compared using comparative proteomic and bioinformatics technology. This research will bulid an experimental basis for the establishment of quinquefolium L. quality evaluation standard system in protein level, provide a new method for the identifying in different parts of quinquefolium L. root and also provide a scientific theory for the development and utilization of resources.Purpose: To establish two-dimensional electrophoresis system, analysis the 2-DE maps in different parts of quinquefolium L. and explore part of the protein function, thereby providing method and experimental basis for the improvementof quinquefolium L. internal quality evaluation standard system in protein level.Method: In this research, four years quinquefolium L. was used as experimental meterial. First, the optional quinquefolium L. protein extraction method was screened among direct extraction, acetone precipitation and majorized TCA/acetone precipitation method, and then the parameters of 2-DE apparatus were groped for establishing the 2-DE system for which suitable quinquefolium L. proteomic analysis. Second, the 2-DE maps in different parts of quinquefolium L. root were obtained with 2-DE system established, and then the maps were analysed with Image Master 2D Platinum Version 6.0, thus the special and different expressed protein spots were detected. Thrid, as well the last, made an initial research to some detected proteins using mass spectra (MS) technique and protein databases.Results: First, majorized TCA/acetone precipitation method which was the optimized quinquefolium L. protein extraction method was established. Second, the 2-DE system of quinquefolium L. root were established; High resolution and repeatable 2-DE maps in different parts of quinquefolium L. root were obtained and the six special (lateral root S9, rhizome S10, main root S11-14) and 17 pairs of different expressed protein spots were detected. Third, the ininital MS identification results showed that all the identified proteins could be divided into five different groups, they are respectively response to stress related proteins (S1,S2), energy related proteins (S4,S5), storage related proteins (S7,S10,S12-14), predicted proteins (S9) and unkown proteins (S11). The comprehensive and systematic identification of different parts of quinquefolium L. root is underway. Conclusion: In this paper, two-dimensional electrophoresis (2-DE) system for which suitable quinquefolium L. proteomic analysis was established. By comparing the 2-DE maps of different samples, special and different expressed proteins were detected,and following, the founction of partical detected proteins were identified by MS and bioinformatics technology. This research will provide experimental basis and method guiding for the establishment of quinquefolium L. internal quality evaluation standard system, and also provide a powerful theoretical basis for the identifying in different parts of quinquefolium L. root, and then lay a foundation to drive the development of quinquefolium L. industry.
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