Analysis Of SDS-PAGE Protein Electrophoresis In Cerebral Cortex Of Rats After Whole Body Deep Hypothermic Circulatory Arrest

[Objective] To explore the effect of deep hypothermic circulatory arrest (DHCA) on expression of related molecular weight range proteins in cortex neuron of rats.[Methods] Fourty-eight SD rats were divided randomly into three groups:deep hypothermic circulatory arrest (DHCA) group, normal temperature circulatory arrest (NTCA) group and normal control (NC) group. The animal model of closed-chest DHCA was established in rats by means of external cannulation. In DHCA group, circulatory arrest10min under the deep hypothermia (20℃) after cardiopulmonary bypass; in NTCA group, circulatory arrest lOmin under the normal temperature (36.8℃～37℃) after cardiopulmonary bypass; in NC group, cardiopulmonary bypass20mins under the normal temperature(36.8℃～37℃)after cardiopulmonary bypass without circulatory arrest and deep hypothermia. Then cerebral cortexes were removed quickly and the specimens were stored in liquid nitrogen. Use SDS-PAGE way to measure related protein molecular weight range.[Results] The level of43-55KDa molecular weight range proteins expression of DHCA group and NTCA group than in the control group was significantly increased, the difference was statistically significant (P<0.05), The level of43～55KDa molecular weight range proteins expression of DHCA group than in the NTCA group was significantly increased, the difference was statistically significant (P<0.05)[Conclusion] Ischemia can induce the expression of43-55KDa molecular weight range proteins, and deep hypothermia could increase43-55KDa molecular weight range proteins expression.The increased expression of43～55KDa molecular weight range proteins could be one of the mechanisms of deep hypothermia brain protection.