| Objective To explore the effectiveness of topiramate on brain edema, brain injured volume and NSE serum levels after TBI in rats.Method 1. Experimental traumatic brain injury was induced in rats with Feeney's model.45 adult Wistar rats were randomly divided into sham operation group (15):only emerged the dura but not injured; control group (15):injured the brain but not treated;TPM treated group (15):after TBI were treated with TPM. After TBI, TPM (TPM treated group) or sodium chloride (sham operation group and control group) were administered immediately and once a day.2. Neuron-specific enolase levels in serum:Twenty-four hours after traumatic brain injury, blood was elicited by cardiac. Serum was separated by centrifuge. Neuron-specific enolase in serum was measured by radioimmunoassay.3. Detection of brain water content:The brains were removed and were measured with the wet-dry method 48 hours after TBI.4. Evaluation of brain injured volume:three days after traumatic brain injury, the brain injured volume was evaluated through TTC staining.Result 1. Neuron-specific enolase levels in serum were (2.20+0.25) ng/ml in the sham operation group;(9.76±1.18) ng/ml in the control group; (5.81±2.27) ng/ml in the TPM treated group. NSE serum levels increased obviously in both the TPM treated group and the control group comparing with that in the sham operation group (P＜0.05). NSE serum level was significantly lower in the TPM treated group than that in control group (P<0.05).2. Brain water content was (70.5±0.5)% in the sham operation group; (91.6±0.6)% in control group; (80.2±0.5)% in TPM treated group. The brain water content increased obviously in both the TPM treated group and the control group comparing with that in the sham operation group (P＜0.05). The brain water content reduced significantly in TPM treated group comparing with that in the control group (P＜0.05).3.The brain injured volume was 0 in the sham operation group; (37.0±2.6) mm3 in the control group; (18.52.5) mm3 in the TPM treated group. The brain injured volume increased obviously in both the TPM treated group and the control group comparing with that in the sham operation group. (P＜0.05). The brain injured volume reduced significantly in the TPM treated group comparing with that in the control group (P＜0.05).Conclusion These data suggested that topiramate neuroprotective effects on traumatic brain injury in rats model, and that the mechanism of neuroprotection may relate to reduce neuron damage,brain edema and brain injured volume.
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