Studies On Chemical Constituent And Antioxidation Activity Of Orobanche Cumana Wallr·

Orobanche cumana Wallr·mainly distributed in heilongjiang,jilin,liaoning,neimenggu,hebei,shanxi, beijing,gansu,qinghai and xinjiang province,It was rich in resources.Sum up related Literatures,we found it was fewer about O.cumana Wallr·in aspect of chemical constituent and antioxidation research,which limited development and utilization of this resources. In this paper, chemical constituent and components of antioxidant activity of O.cumana Wallr·were system studied,providing theoretical basis for further development of Orobanchaceae medicinal plants.The main conclusions as follows:1,Using DPPH method for O.cumana Wallr·aqueous two-phase extraction of petroleum ether,dichloromethane,ethyl acetate,n-butanol four compositions of Antioxidant Activity research.The results showed that each polar extraction fractions of O.cumana Wallr·had scavenging capacity to DPPH radical. IC50 of each fraction of O.cumana Wallr·were respectively: petroleum ether(0.9256mg·mL-1);dichloromethane(6.0339mg·mL-1);ethyl acetate(0.2542mg·mL-1);n-butanol(0.2213mg·mL-1).As can be seen that ethyl acetate and n-butanol fraction were obviouly,Identified as the active components.2,The chemical constituent of O.cumana Wallr·were separated and purificated by silica gel,Sephadex LH-20 chromatographic techniques.Eleven compounds were isolated from O.cumana Wallr·.Among them, Six compounds were identified and isolated from O.cumana Wallr·for the first time,Respectively:crenatoside (Ⅰ),isocrenatoside(Ⅱ),sitosterol (Ⅲ),stigmasterol(Ⅳ),daucosterol(Ⅴ),mannitol(Ⅵ).With screening tests of structuralidentificationthe and antioxidant activity,The results showed that crenatoside and isocrenatoside phenylethanoid glycosides which were separated from active components had stronger antioxidative.It was the main component of antioxidative.