The Mechanisms Of Oridonin In SGC7901 Cells

Objective::To explore the mechanism of potential anticancer effect of oridonin and lay the foundation for the further development and utilization of diterpenoid anticancer drugs.Methods:(1) MTT assay was used to evaluate cell growth inhibitory rate. (2) The change of cell morphology was observed through microscope observation;Apoptosis was analyzed by Hoechst33342 fluorescence staining.(3)Apoptosis ratio and cell cycle distribution were analyzed by flow cytometry.(4) Western blot was applied to detecte the expressions of NF-κB,p-JNK protein,and the expressions of p-JNK after PDTC inhibiting NF-κB activation.Results:(1)MTT result shows that Oridonin could inhibited SGC7901 cells in dose-and time-dependent manner. It presented the IC50 values were(21.43±2.16)μg/L at 24 h (P<0.05),and when treated with 40μM at 60h, the inhibit value was 92.92±3.31%(P<0.05).(2) Oridonin could inbibit SGC7901 growth obviously,the inverted microscope showed cells firstly became sparse,rounded,and then detached from the dishes.(3) Corresponding, Hoechst staining analysis was also used to further determine the apoptosis, the result showed typical apoptotic morphological changes, including cytoplasmic blebbing,condensation and aggregation of nuclear chromatin and the formation of apoptotic bodies, compared with the uniform staining of control group.(4) Flow cytometry showed that oridonin caused a dose-dependent increase cell number of cells in the G0/G1 phase in a way of dose-dependent and decreased cell number in G2/M, which indicated that oridonin caused G0/G1 arrest in SGC7901 cells.After treatment with various concentrations of oridonin, significant apoptotic peak appeared.(5)Orindonin inhibited the expression of NF-κB and p-JNK protein in dose-dependent way. (6)After treated with the inhibitor PDTC of NF-κB,SGC7901 expressed the p-JNK protein lowly.Conclusion:Oridonin could inhibit SGC7901 proliferation through inducing cell apoptosis and cell cycle inhibition at GO/G1.The effect might be that oridonin could directly or indirectly downregulate NF-κB,p-JNK protein,and NF-κB mediated the activation of p-JNK.