Study On Morphology And Function Of Orthotopic And Subcutaneous Fetal Ovarian Transplantation In Rats

Objective:To explore the growth and development of fetal ovary in rats after orthotopic and subcutaneous transplantation,to establish the animal models of fetal ovary transplantation in situ, in order to study restoration of natural fertility after transplantation and safety of the offspring.Methods:SD rats of 40-50 days old were divided into four different groups randomly which were Orthotopic transplantation group,subcutaneous transplantation group,positive control group and negative control group,while the fetal rats of 18-19 days were ovary donors.Each group got different operations.After transplantation,Estradiol and progesterone level were tested,the growth and development of fetal ovary were observed at the same time.Results:Ovaries developed well after transplantation, all stages of follicles and corpus luteum were observed.The estradiol level(x±s)of each groups were:107.4±10.02pmol/L,106.73±9.67pmol/L, 111.81±4.36pmol/L,46.58±7.74pmol/L, and progesterone level(x±s) were:11.86±2.39ng/ml,11.60±1.13ng/ml,12.06±0.72ng/ml, 3.02±0.64ng/ml.There was no statistical difference among orthotopic transplantation group,subcutaneous transplantation group and positive control group (P>0.05),while there was statistical difference between transplantation groups and negative control group(P<0.01).Conclusion:1.Fetal ovary can growth and develop well after orthotopic transplantation.2.Restoration of endocrine function after orthotopic transplantation were similar with subcutaneous transplantation.3.It's feasible to establish animal models of fetal ovary transplantation in situ,in order to study the restoration of natural fertility after transplantation and safety of progeny.