Effects Of Gastrin And Its Antagonist On Cell Proliferation And The Expression Of HB-EGF In Human Gastric Cancer Line MKN45

Objective:To explore the effects of gastrin-17 and its antagonist PGL on cell proliferation and the expression of HB-EGF in human gastric cancer line MKN-45. Methods:MKN45 cells were incubated in the medium with gastrin-17 (10-6～10-10mol/L),proglumide(10-2～10-4mol/L) and the combination of these two agents (10-8mol/L gastrin-17 and 10-2～10-4 mol/L proglumide),respectively.Cell growth and proliferation of MKN45 were analyzed with MTT assay,expression of HB-EGF was determined by Western-Blot. Results:MTT showed gastrin-17 significantly promote cell proliferation at the concertration of 10-6～10-9mol/L (P<0.05),PGL at the concertration of 10-2～10-3mol/L significantly inhibite the proliferation of MKN45 cells (P<0.05). In the combination of these two agents, PGL (10-2～10-3mol/L) significantly blocked the proliferation of MKN-45 cells induced by gastrin-17 (P<0.05). Meanwhile, WB showed gastric cancer line MKN45 have the expression of HB-EGF protein. Gastrin-17 at the concertration of 10-6～10-9mol/L significantly strengthen the expression of HB-EGF in MKN45 cells (P<0.05). In the combination of these two agents, PGL (10-2～10-3mol/L) significantly blocked the expression of HB-EGF stimulated by gastrin-17(P<0.05).Conclusions:Gastrin-17 can promote cell proliferation in human gastric cancer line MKN45, its antagonist PGL significantly blocked the role. PGL significantly inhibite the proliferation of MKN45 cells.Gastric cancer line MKN45 have the expression of HB-EGF protein.Gastrin-17 significantly strengthen the expression of HB-EGF, its antagonist can blocked the expression of HB-EGF stimulated by gastrin-17.