Abnormal Expression Of WWOX Gene In Human Benign And Malignant Pleural Effusion

Objective:To detect the abnormal expression of WWOX (WW domain containing oxidoreductase) exons 6-8 In human lung benign and malignant pleural effusion at mRNA leval and to draw some conclusion concering whether loss of WWOX exons 6-8 play an role in the tumorigenesie of malignant pleural effusion.Methods:76 pleural effusion samples come from Tianjin Chest Hospital Medical Department in 2007 September to 2008 June in, According to cytology results samples were divided into two groups:20 in benign effusions and 56 in malignant effusions which from lung cancer. Take 10ml of pleural effusion, extracted total RNA, react cDNA by RT, after PCR 5μl of sample electrophoresis, observe and photographed in ultraviolet light. To analysis the relationship of the expression of WWOX exons 6-8 with clinical charactors (gender, smoking, histological type, stage),we use chisquare test and exact probability test of contingency table by spss 13.0 sofeware, P<0.05 have statistics difference.Results:(1) malignant effusions groups,39 (69.6%) specimens failed PCR amplification of DNA fragments of the expected length,in 56 cases of malignant pleural effusion,39 specimens failed to amplify exon 6-8 WWOX gene fragment, while the samples of 20 (100%) patients with benign pleural effusions WWOX gene was amplified exon 6-8 fragment (χ2 is 6.765,p<0.05).31 cases of pleural effusion by pleural fluid cytology and pathological diagnosis of lung cancer sediment samples cannot amplificate exon 6-8 WWOX gene fragments, and pleural fluid cytology and pleural effusion sediment pathology negative.Finally, in 25 cases of malignant pleural effusion of lung cancer samples confirmed by pleural biopsy,8 cases failed to amplify exon 6-8 WWOX gene fragments.(2) We further observed the transcript loss of WWOX exons 6-8 was significantly higher in the men compared to the women (p=0.001), it is also higher in the smokers compared to the none-smokers (p=0.001)and squamous cell carcinomas compare to adenocarcinomas(p=0.041), But it was not associated with TNM stage(p=0.27). There is higher transcript loss of WWOX exons 6-8 in male,heavily smoking,non small cell lung cancer patients.Conclusion:The study shows that, at 16q23.3-24.1 of the WWOX gene in non-small cell lung cancer there is a higher transcript exon 6-8 deletion rate,it suggests that WWOX gene may play an important role in the development of malignant pleural effusion,the loss of WWOX gene transcripts exon 6-8 is an molecular marker of malignant pleural effusion; WWOX gene exon 6-8 mRNA can be used as indicators in identifying benign and malignant pleural effusion.