Study On The Regulating Mechanism Of Electro-acupuncture On Glutathione Antioxidant System Of The Cerebral Ischemia-reperfusion Model Rats

Objective:By observing that electro-acupuncture impacts on neurological behavioral score and TTC staining of model rats with the middle cerebral artery with focal cerebral ischemia-reperfusion injury, as well as, the impact on reduced glutathione (GSH) content, oxidized glutathione (GSSG) concentrations, glutathione peroxidase (GSH-Px) activity and GSH/GSSG relative ratio of its liver and cerebral cortex tissue by stimulating to BaiHui and DaZhui acupoints, so as to assess the anti-oxidation of acupuncture on model rats with cerebral ischemia-reperfusion injury, further explore the antioxidant stress mechanism of the acupuncture in treating ischemic stroke, and provide a experimental basis for clinical prevention to the cerebrovascular disease.Methods:The 39 clean-level SD male rats (300±20g) were randomly divided into 3 groups:sham operation group, model group, EA group, each 13. Adoption of model rats with the middle cerebral artery with focal cerebral ischemia-reperfusion injury, stimulation to BaiHui and DaZhui acupoints by electro-acupuncture (continuous wave, frequency:3 Hz, current intensity:1-3mA, voltage:1-3V,30min), observation the neurological behavioral score and TTC staining, and test the GSH concentration, GSSG concentration, GSH-Px activity and GSH/GSSG relative ratio in the cerebral cortex and liver of rats each group after reperfusion 24h, using statistical software SPSS 13.0 for statistical analysis.Results:1. Compared with the sham-operated group, the neurological behavioral score of the model group and electro-acupuncture group were significantly lower (P<0.01), while the score after the electro-acupuncture was significantly higher than that of the model group (P<0.01).2. Compared with the sham-operated group, the TTC staining of model group has apparent white infarction at ipsilateral cerebral hemisphere, while the white area of the electro-acupuncture group has reduced compared with model group.3. Compared with the sham-operated group, the GSH content significantly reduced, GSSG concentration significantly higher, GSH/GSSG relative ratio significantly reduced, and GSH-Px activity significantly reduced in liver and cerebral cortex tissue of model group rats, these differences have a very significant statistical difference (P<0.01). While Electro-acupuncture can significantly promote the reduced content of GSH to increase, the elevated concentration of GSSG to decrease, increase the relative ratio of GSH/GSSG, and regulate the GSH-Px activity.Conclusion:1. The neurological behavioral score and TTC staining results showed that brain damage of electro-acupuncture group rats significantly lighter than that of the model group, indicating that electro-acupuncture can reduce brain damage and improve neurological behavioral deficit of rats caused by ischemia-reperfusion injury.2. Electro-acupuncture can significantly promote the reduced content of GSH to increase, the elevated concentration of GSSG to decrease, increase the relative ratio of GSH/GSSG, and regulate the GSH-Px activity in the tissue of the ischemia-reperfusion injury rats, indicating that electro-acupuncture can adjust the function of glutathione antioxidant system to close to the equilibrium condition.3. Acupuncture has the antioxidant effects. Electro-acupuncture at BaiHui and DaZhui acupoints may improve neurological behavioral deficit, rectify the imbalance of GSH and GSSG and adjust the function of glutathione antioxidant system in liver and cortical tissue of rats suffered with cerebral ischemia, so that it can play an effect in protecting brain nerve cells, adjusting the cerebral circulation and improving the function of ischemic nerve cell.