The Kidney Hemodynamics Changes And It's Relationship Of Renal Tissue MPO Were Evaluated By The Use Of Color Doppler Ultrasound In Rabbits With Renal Ischemia-reperfusion Injury

objective:By the use of color Doppler ultrasound, the kidney hemodynamics changes and it's relationship of renal tissue MPO were investigated in rabbits with renal ischemia-reperfusion injury. Methods:56 rabbits were randomly divided into normal control group (n=8),sham operation group (n=24),ischemia-reperfusion group(n=24),and sham operation group and ischemia-reperfusion group were also divided into into 2h,8h,24h 3 subgroups(each n=8) according to reperfusion time points.Each group of rabbits were anesthesied with 2% sodium pentobarbital through the ear vein injection(dosage 1.5ml/kg).After the success of anesthesia, the hair of left kidney area was removed and try skin to exposed. No surgery in control groups, the right kidney were all removed both sham operation and ischemia-reperfusi-on groups,the renal arteriovenous were occlusioned with the artery clamp,and resulted in renal ischemia, after renal ischemia for 1h, and restoration of perfusion were recovered. By the use of CDFI, left renal portal master renal artery,renal sinus segment artery,renal interlobar artery between bilateral vertebral margin of leaf,three levels of renal blood flow dynamics were monitored in each group,and detected the artery peak systolic velocity (Peak systolic velocity Vmax),end diastolic velocity(End-diastolic velocity Vd),time average peak velocity(Time average peak velocity Tamax),pulsatility index (Pulsatility index PI)and resistance index(Resistance index RI).Determination of renal arterial blood flow in each group had been finished,the left kidney were removed in the low temperature state,left kidney tissue were weighed 100mg,which were used to measure the renal tissue MPO activity by MPO kit. The hemodynamic parameters were tested through homogeneity of variance,and undertaking ANOVA analysis(P<0.05 was regared as statistically significance)；three levels renal artery Vmax,Vd,Tamax,PI,RI and the relationship between MPO activity were analyzed by linear correlation and regression to calculate the correlation coefficient r value(P<0.05 as statistically significant).Results:(1)Hemodynamics changes are as follow:①Normal control group:master renal artery Vmax,Vd,Tamax,PI,RI were 42.50±4.35 (cm/s),24.88±3.96(cm/s),29.75±4.17(cm/s),0.56±0.12,0.41±0.07；segmental artery Vmax,Vd,Tamax,PI,RI were 28.79±6.33(cm/s),18.12±3.41(cm/s),21.09±3.62(cm/s),0.48±0.12,0.37±0.07；interlobar artery Vmax,Vd,Tamax,PI,RI were 18.21±2.89(cm/s),12.57±3.72(cm/s), 13.89±3.76(cm/s),0.46±0.09,0.34±0.05.②Sham operation 2h subgroup: master renal artery Vmax,Vd,Tamax,PI,RI were 43.20±5.65(cm/s),23.85±4.37(cm/s),29.81±5.11(cm/s),0.58±0.08,0.42±0.06;segmental artery Vmax,Vd,Tamax,PI,RI were 28.98±7.56(cm/s),16.91±4.32(cm/s), 20.17±4.21(cm/s),0.50±0.05,0.38±0.08；interlobar artery Vmax,Vd, Tamax,PI,RI were 19.43±5.89(cm/s),12.86±3.14(cm/s),14.12±3.87 (cm/s),0.47±0.13,0.35±0.07；Compared with the normal control group, sham operation 2h subgroup of master renal artery,segmental artery,interlobar artery Vmax,Vd,Tamax,PI,RI change were not significantly different (P>0.05).③Sham operation 8h subgroup:master renal artery Vmax,Vd, Tamax,PI,RI were 41.74±7.20(cm/s),22.97±5.14(cm/s),28.51±6.21 (cm/s),0.57±0.10,0.41±0.10；segmental artery Vmax,Vd,Tamax,PI,RI were 27.12±6.81(cm/s),16.22±5.73(cm/s),19.89±4.74(cm/s),0.49±0.07,0.37±0.10；interlobar artery Vmax,Vd,Tamax,PI,RI were 18.34±5.17 (cm/s),12.62±4.62(cm/s),13.97±4.23(cm/s),0.46±0.11,0.35±0.11；respectively Compared with the normal control group,sham operation 2h subgroup,sham operation 8h subgroup master renal artery,segmental artery, interlobar artery Vmax,Vd,Tamax,PI,RI change were not significantly different(P>0.05).④Sham operation 24h subgroup:master renal artery Vmax, Vd,Tamax,PI,RI were 42.98±5.44(cm/s),23.58±7.08(cm/s),29.22±6.38(cm/s),0.57±0.11,0.42±0.08；segmental artery Vmax,Vd,Tamax,PI, RI were 28.65±7.43(cm/s),17.56±6.13(cm/s),20.84±5.12(cm/s),0.49±0.10,0.38±0.06；interlobar artery Vmax,Vd,Tamax,PI,RI were 19.71±3.57(cm/s),13.32±4.54(cm/s),14.37±4.69(cm/s),0.47±0.09,0.35±0.08.Compared with the normal control group,sham operation 2h and 8h subgroup,sham operation 24h subgroup master renal artery,segmental artery, interlobar artery Vmax,Vd,Tamax,PI,RI change were respectively not significantly different(P>0.05).⑤Ischemia-reperfusion 2h subgroup:master renal artery Vmax,Vd,Tamax,PI,RI were 46.23±4.53(cm/s),24.36±6.19 (cm/s),31.41±4.91(cm/s),0.67±0.18,0.47±0.14；segmental artery Vmax, Vd,Tamax,PI,RI were 30.40±3.15(cm/s),18.52±2.86(cm/s),22.27±2.66(cm/s),0.61±0.10,0.44±0.09；interlobar artery Vmax,Vd,Tamax,PI, RI were 20.65±3.83(cm/s),11.92±3.27(cm/s),13.42±3.98(cm/s),0.59±0.08,0.40±0.07；Compared with the normal control group,sham operation the subgroups,ischemia reperrusion 2h subgroup master renal artery,segmental artery,interlobar artery Vmax,Vd,Tamax,PI,RI change respectively were not significantly different(P>0.05).⑥Ischemia-reperfusion 8h subgroup:master renal artery Vmax,Vd,Tamax,PI,RI were 48.17±7.05(cm/s),24.88±6.79 (cm/s),33.31±6.00(cm/s),0.75±0.19,0.49±0.08；segmental artery Vmax, Vd,Tamax,PI,RI were 34.25±6.18(cm/s),18.36±4.16(cm/s),24.94±3.97(cm/s),0.72±0.16,0.48±0.07；interlobar artery Vmax,Vd,Tamax,PI, RI were 23.26±5.25(cm/s),11.69±3.84(cm/s),15.19±4.42(cm/s),0.70±0.18,0.45±0.10.Compared with the normal control group,sham operation the subgroups,ischemia-reperfusion 8h subgroup master renal artery Vmax,Vd, Tamax,PI,RI change respectively were not significantly different(P>0.05)；segmental artery,interlobar artery RI increased(P<0.05),while the Vmax,Vd, Tamax,PI did not differ significantly(P>0.05).⑦Ischemia-reperfusion 24h subgroup,master renal artery Vmax,Vd,Tamax,PI,RI were 55.11±4.34(cm/ s),20.60±7.87(cm/s),31.79±7.10(cm/s),1.09±0.54,0.63±0.13； segmental artery Vmax, Vd, Tamax, PI, RI were 37.71±3.53 (cm/s),17.67±2.99 (cm/s),24.47±3.25 (cm/s),0.97±0.14,0.57±0.11;interlobar artery Vmax,Vd,Tamax,PI,RI were 28.66±5.81(cm/s),9.73±2.97(cm/s), 13.88±3.91(cm/s),0.89±0.32,0.54±0.09;Compared with the normal control group, sham operation subgroups, ischemia-reperfusion 24h subgroup master renal artery, segmental artery, interlobar arteries Vmax, PI and RI increased (P<0.05),while Vd and Tamax respectively did not differ significantly (P> 0.05).(2) MPO activity in renal tissue:the normal control group,MPO activity was 0.14±0.11U/g.Sham operation 2h,8h,24h subgroups MPO activity were 0.15±0.10U/g,0.15±0.12U/g,0.16±0.07 U/g, compared with the control group, Sham operation 2h,8h,24h subgroups MPO activity had no significant difference (P>0.05).Ischemia-reperfusion 2h, 8h,24h subgroups MPO activity were 0.34±0.17U/g,0.46±0.15U/g,0.77±0.22 U/g, compared with the control group and Sham operation 2h,8h,24h subgroups, Ischemia-reperfusion 2h,8h,24h subgroups MPO activity were significantly higher (P<0.05).(3)Ⅰ:The linear correlation analysis:①The master renal artery Vmax, PI, RI and renal tissue MPO activity was positively correlated(r=0.431,0.639,0.578,P<0.05),and Vd, Tamax are no significant correlation (P>0.05).②The renal segmental artery of the Vmax, PI, RI and renal tissue MPO activity was positively correlated(r=0.570,0.536,0.633, P<0.05), and Vd, Tamax had a significant correlation (P>0.05).③The renal interlobar artery Vmax, PI, RI and renal tissue MPO activity was positively correlated (r=0.403,0.467,0.475,P<0.05), and Vd, Tamax had a significant correlation (P>0.05).Ⅱ:The linear regression analysis:①MPO activity for each group would be variable as y, to master renal artery of Vmax as the independent variable x, for linear regression analysis, linear regression equation: y=0.019x-0.539.②MPO activity for each group would be variable as y, with master renal artery PI as the independent variable x, for linear regression analysis, linear regression equation:y=0.525x-0.054.③MPO activity for each group would be variable as y, to master renal artery of RI as independent variable x, for linear regression analysis, linear regression equation: y=1.288x-0.306.④MPO activity for each group would be variable as y, to segmental artery of Vmax as the independent variable x, for linear regression analysis, linear regression equation:y=0.026x-0.517.⑤MPO activity for each group would be variable as y, in order to segment artery PI as the independent variable x, for linear regression analysis, linear regression equation: y=0.516x-0.010.⑥MPO activity for each group would be variable as y, in order to segment artery RI as the independent variable x, for linear regression analysis, linear regression equation:y=1.466x-0.326.⑦MPO activity for each group would be variable as y, in interlobar arteries of Vmax as independent variables x, for linear regression analysis, linear regression equation: y=0.019x-0.115.⑧MPO activity for each group would be variable as y, to the PI of interlobar arteries as the independent variable x, for linear regression analysis, linear regression equation:y=1.232x-0.196.⑨MPO activity for each group would be variable as y, to the RI of interlobar arteries as the independent variable x, for linear regression analysis, linear regression equation: y=0.549x-0.022.Conclusions:(1)Rabbit renal artery of renal ischemia-reperfusion within 24h hemodynamics has change, and vascular tissue at the end change first appeared, using CDFI to monitor rabbit renal arterial hemodynamics of renal ischemia-reperfusion within 24h is a effective way. (2) After ischemia-reperfusion 2h Rabbit renal MPO activity was significantly increased,and gradually increasing trend within 24h, indicating renal ischemia-reperfusion within 24h leukocyte aggregation increased, kidney inflammation response, suggesting the persistence of renal tissue injury. (3) From rabbit kidney within the renal ischemia-reperfusion 24h master renal artery, segmental artery, interlobar artery Vmax,RI, PI and MPO activity was positively correlated,can be speculated that the event of kidney perfusion abnormalities,there is the situation of ischemia and reperfusion,in the early 24h CDFI monitor arterial blood flow having significantly change,kidney damage should be considered, CDFI is a non-invasive diagnosis of renal ischemia-reperfusion injury feasible method.