| Objective:After aspirin (ASA) pretreatment,the changes dyna-mically of the ischemic brain tissue IL-6 (IL-6) and cytokine signal transduction inhibitor-3 (SOCS-3) mRNA expression are studied after rat focal cerebral ischemia/reperfusion (CI/RP), so as to clarify the role of aspirin, the possible mechanism of brain protection. Methods:95 male healthy SD rats were randomly divided into sham-operated group, ischemic control group, low-dose ASA group (10mg.kg-1) and high-dose ASA group (150mg.kg-1). Modified suture method is used rat focal cerebral ischemia/ reperfusion model. Each rat for 7 days before modeling their different doses of ASA or vehicle gavage,1/d. Administered 1h before surgery and then 1. After at 6h,12h,24 h,72h,7d time rats were killed immediately and tested the corresponding cranial indicators of brain,5 rats at each time point. Another 5 rats in each group also were killed 24h after the operation, for TTC staining and infarct volume. At each time point neurological score. Taking ischemic cerebral hemisphere is measured in brain tissue water content in brain tissue, IL-6 and SOCS-3mRNA content, and the pathological staining.Results:1A rat MCA-CI/RP after the control group, neurological deficits of different degrees, the water content in cerebral infarction increased, both in the 24h peak, TTC staining can clearly show the scope of infarct lesion, HE staining lesion side shows the performance of ischemic brain tissue.2 ischemic side IL-6, SOCS-3mRNA dynamic changes:6h after ischemia and reperfusion IL-6 expression compared with sham group the expression of IL increased (P<0.05),24h after the peak, then gradually decreased,7 days remains slightly high. RT-PCR determination of SOCS-3 mRNA shows the same regularity.3 ASA on the above indexes:(1) CI/RP post-operative, two experimental Neurological score is significantly higher than the control group, nerve function can be improved to varying degrees, while the surrounding brain tissue with infarct reduce the amount of water, small doses of ASA group compared with the control group after 12h a significant difference appears to 7d after the two groups compared with the control group was significantly different(P<0.05); (2) Small, high-dose ASA group after 24h infarct lesion volume decreased by 54.48%,30.90%, compared with the control group.(3)It was less that extent of ischemic necrosis of neuronal cells of HE staining ASA10mg.kg-1 group of lesions in brain tissue, intercellular part of the widened. It was severe that extent of ischemic necrosis of neuronal cells of ASA150mg.kg-1 group of lesions of neurons in brain tissue, cell gap widened more dramatically; were better than the control group. (4) CI /RP post-operative, two experimental group's side of the brain lesions IL-6 levels decreased significantly compared with the control group,12h after the operation to the 3d compared with the control group there were significant differences(P<0.05). (5) CI/RP post-operative, two experimental groups side of the brain lesions SOCS-3mRNA levels significantly increased compared with the control group,12h after the operation to the 3d compared with the control group there were significant differences(P<0.05). Conclusions:(1) improved cerebral ischemia-reperfusion model is a simple preparation, high success rate, favorable pharmacological preconditioning of the model. (2) brain injury after cerebral ischemia mainly in neurological function score decreased brain water content increased, cerebral infarction and brain abnormalities HE staining, and IL-6, SOCS-3mRNA in the CI/RP patients increased relationship with the indexes in aging, suggesting that inflammation and brain edema formation and development of neurological deficits caused by one of the main, while SOCS-3mRNA may play an endogenous neuroprotective agent. (3) ASA drugs can reduce the MCA-CI/RP pretreatment neurological deficits, reduce brain water content; reduced cerebral infarct volume; improve HE staining of brain tissue pathological changes. The possible mechanism and inhibition of inflammatory cytokines IL-6 production and increases SOCS-mRNA related. (4)With the increase of ASA's dose the neuroprotective effect is not increase; low dose of ASA is superior to high-dose ASA group... |
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