Study On Alterations In Gallbladder Pathology, NF-κB Expression In Gallbladder Tissues, Et, IL-8 In Gallbladder Tissues And Serum Between Obstruction Of Common Bile Duct And Its Relief

Objective: In this study, rabbits with acute common bile duct(CBD) obstruction are prepared as subjects, alterations of gallbladder pathology, NF-κB expression in gallbladder tissues, ET, IL-8 in gallbladder tissues and serum of CBD obstruction at different times and after obstruction relief are observed for providing animal experiments and theoretic basis for clinic treatment of acute cholecystitis caused by CBD obstruction.Methods: 54 healthy rabbits, weighing 2.2-2.5kg,in half respectively male and female, were randomly divided into nine groups, six in one group: normal control group (N); common bile duct obstruction group (B), in which group B1 for obstruction one day, group B3 for obstruction three days, group B5 for obstruction five days, group B7 for obstruction seven days; obstruction relief group (R), in which group R1 for relief after one day, group R3 for relief after three days, group R5 for relief after five days, group R7 for relief after seven days. Before operation, each rabbit is provided water, no food. The next day, after laparotomy, the CBD end of each rabbit is separated. For group N, the rabbits are put to death after 72 hours. For rabbits in group B and group R, obstruction is prepared. For the rabbits in group B, put them into death after obstruction for 1 day, 3days, 5days and 7 days respectively; for group R, put them into death 72 hours after obstruction relief. Blood is drawn from heart for all rabbits and serum is separated, and IL-8 and ET in serum and gallbladder tissue are measured by radio-immunity method. Gallbladder pathology is observed by HE method and NF-κB expression in gallbladder tissues is observed by immunohistochemical method. The experimental data is showed in mean±standard deviation. The statistic significance is analyzed by SPSS 13.0 a statistic sofeware. The mean of each group is analyzed by one-way ANOVA and inspected by Student-Newman-Keuls. P<0.05 is found to have statistic significance.Results:General conditions and pathology of the gallbladder: The obstruction of common bile duct can induce acute inflammation of gallbladder. Compared with group N, the gallbladder volume of rabbits in group B1 and B3 becomes larger and the gallbladder wall becomes thicker and edema, while the gallbladder wall is integrated and has no ulcer, and the bile is in deep yellow; under the microscope, the mucosa is integrated, each layer has edema, the blood vessels are congested and a few inflammatory cell soaks. The gallbladder volume of rabbits in group B5 and B7 has a significant dilation, ulcers even perforation can be seen at the bottom of gallbladder mucosa, and the bile is turbid and dark green mostly; stone can be seen in gallbladder in group B7. Under the microscope, the mucosa desquamates partially, and a large number of inflammatory cells soak in each layer in group B5; the mucosa disappears, many dead inflammatory cells can be seen in gallbladder tissues in group B7. Inflammation is decreased in group R compared with group B in the same period.NF-κB expression in gallbladder tissues: the NF-κB expression is enhanced with the obstruction days, a large number of nuclear stained cells can be seen in group B7, the number of positive cells and the NF-κB expression in group R are both decreased compared with group B in the same period.ET, IL-8 in gallbladder tissues and serum: the content of ET, IL-8 in gallbladder tissues and serum increases gradually with the obstruction days, there is statistic significance among group B1, B3, B5 and B7 (P<0.05); the content of ET, IL-8 in gallbladder tissues and serum decreases in group R compared with group B in the same period, having statistic significance (P<0.05).Conclusions: 1. Common bile duct obstruction can induce acute cholecystitis. Gallbladder inflammation is increased gradually with the increasing of obstruction time. Cholecystitis can be alleviated with the relief of obstruction.2. The NF-κB expression is enhanced with the worsened cholecystitis. NF-κB, as an important transcription factor involved in inflammation, also has a close relationship with cholecystitis3. With the increasing of obstruction time, gallbladder inflammation is increased gradually, even spreading through the body, the content of IL-8 in gallbladder tissues and serum increases gradually; with the relief of obstruction, cholecystitis can be alleviated and the content of IL-8 in gallbladder tissues and serum decreases. IL-8, as an important cellular factor, may reflect the gallbladder tissues and systemic inflammatory response.4. With the increasing of obstruction time, the content of ET in gallbladder tissues and serum increases gradually; with the relief of obstruction, the content of ET in gallbladder tissues and serum decreases. ET, as an important factor involved in tissue injury, also plays a role in the process of gallbladder damage which caused by the common bile duct obstruction.