Study On Anti-HBV Active Substance And The Content Analysis Of Ampelopsis Megalophylla Diels Et. Gilg

Ampelopsis megalophylla Diels et.Gilg is a medicincal plant of Ampelopsis. It is a herb commonly used in Enshi of Hubei province. It is neutral in nature and has sour and bitter tasting. The article studied on the anti-HBV effective parts and the anti-HBV effective constituents of Ampelopsis megalophylla,and the content of the effective constituent.Ethanol extract of Ampelopsis megalophylla (Ⅰ) was dissolved in water and fracticnated sequentially with Petroleum ether and EtoAc. The fraction were concentrated to yield the Petroleum ether fraction(Ⅱ), EtoAc fraction (Ⅲ) and water fraction (Ⅳ). The TC50 ofⅡ,Ⅲare 99.1μg/ml and 250μg/ml. The IC50 ofⅡto HBsAg and HBeAg are 4.2μg/ml and 26.3μg/ml, the TI are 23.6 and 3.8. The IC50 ofⅢto HBsAg and HBeAg are 16.7μg/ml and 62.5μg/ml, the TI are 14.9 and 4.0.We isolated and purified the chemical contituents in Petroleum ether fraction of Ampelopsis megalophylla. Silica gel column chro-matograph and sephadex LH-20 column chromatograph were used. As a result,4 compounds was isolated and identified. They are Chrysoph-anol, Physcion, Myricetin andβ-sitosterol. The compounds Chrys-ophanol and Physcion are isolated from this plant for the first time. The compounds Chrysophanol, Myricetin from the fractionⅡand Ampelopsin, myricetrin, Quercetin and Quercitrin from the fractionⅢwere used to evaluate the anti-HBV activity in vitro. The Ampelopsin and Myricetin exhibit anti-HBV activity. The TC50 are 187.5μg/ml and 250μg/ml. The IC50 of HBsAg and HBeAg of Ampelopsin are 44.0μg/ml and 15.4μg/ml. The TI are 4.3 and 12.2. The IC50 of HBsAg and HBeAg of Myricetin are 59.3μg/ml and 12.2μg/ml. The TI are 4.2 and 20.5. Ampelopsin and Myricetin are the active compounds from the fractionⅡand the fractionⅢ.HPLC was used to determine the content of Ampelopsin in Ampelop-sis megalophylla from different areas. The result indicated the content of Ampelopsin is about 30% in Ampelopsis megalophylla. This research shows the medicinal plant posseses a broad developing prospect.