Expression Of MIF And VEGF In Cervical Cancer And Associated Research

Objective: This study was designed to observe the expression of Macrophage migration inhibitory factor(MIF) and vascular endothelial growth factor(VEGF) in normal cervical epithelia, cervical intraepithelial neoplasia(CIN) and cervical carcinoma. Furthmore, to analyze their association with the pathological features and their relationship with the development, invasion and metastasis of the cervical carcinomal. And to provide a new thought for diagnosis and therapy of cervical cancer.Method:The expression of MIF and VEGF was investigated by immuno- histoehemistry in 100 cases,including 21cases of normal cervical epithelia(normal cervica group), 28 cases of CIN(CIN group) and 64 cases of cervical carcinoma (cervical carcinoma group).PBS replaced the first antibody as negative control, and positive sections provided by the biological factory were positive control.The expression level of MIF and VEGF in cervical carcinoma was analyzed again clinical pathological factors including ages of patients, pathological type, clinical stage and histological differentiation lymph node metastasis and tumor dimension. Moreover, MIF expression in comparison with VEGF was investigated preliminarily.Results:1. The rate of MIF positive expression inereased from normal cervical epithelia(19.05%) to CIN(42.86%) to cervical cancer(73.44%)(χ2=48.43,P<0.01). Among them, there was statistically significance between cervical cancer and normal cervical epithelia(χ2=36.53,P<0.0125),between cervical cancer and CIN(χ2 = 24.37, P<0.0125),and between CIN and normal cervical epithelia (χ2=18.21, P<0.0125). 2. The rate of MIF positive expression in cervical cancer with stage IIb～Ⅳwas signifieantly higher than that with stage I~IIa(P<0.01) .As well as, It was higher in cervical cancer patients with low differentiation than middle and high differentiation (P<0.01). The difference of MIF positive expression rate was statistically significance between lymph node metastasis and without (P<0.01). But there was no relationship with age of patients, tumor dimension and pathological type (P>0.05).3. The expression of VEGF was 23.81%, 53.57% and 65.63% respectively in normal cervical epithelia,CIN and cervical cancer.The tendency was increased gradually(χ2=41.38,P<0.01).The rate of VEGF positive expression was statistieally difference between normal cervical epithelia and CIN(χ2=17.92,P<0.0125), between normal cervical epithelia and cervical cancer (χ2=15.21,P<0.0125).However, there was no statistically significance between CIN and cervical cancer(χ2=4.58,P> 0.0125).4. The rate of VEGF positive expression in cervical cancer with lymph node metastasis was markedly higher than without(P<0.01).There was no significantly difference between VEGF and other clinical features(P>0.05).5. The expression of MIF and VEGF was positive correlated in cervical cancer(rs=0.784,P<0.05).conclusion:1. The expression of MIF and VEGF was obviously inereased in cervical cancer, and both of the positive expression rate of MIF and VEGF was higher in cervical cancer with lymph node metastasis than without, which hinted that MIF and VEGF may play an important role in carcinogenesis, growth and metastasis in cervical cancer.2.The more later in clinical stage and the more poorer in histological differentiation, the more higher positive expression rates of MIF and VEGF were. The expression of MIF was positive correlated with the expression of VEGF in cervical cancer. Those demonstrated that combined detection of MIF and VEGF may be valuable to evaluate the severity of cervical cancer.