Expression And Clinical Significance Of MIF And VEGF In Bladder Urothelial Carcinoma

Objective To investigate the expression and clinical significance of macrophagemigration inhibitor factor (MIF) and vascular endothelial growth factor (VEGF) inbladder transitional cell carcinoma (BTCC).Methods (1) Seventy-five BTCC tissue samples and adjacent normal tissue samplesobtained from BTCC patients，immunohistochemistry was used to detect the expressionof EGFL7、VEGF and CD34,and determinate its microvascular density(MVD).(2)Meanwhile the relative expression quantity of MIF and VEGF mRNA was assayed byRT-qPCR among75cases of BTCC and adjacent normal tissue samples.(3) MIF、VEGF、CD31、MIF and VEGF mRNA positive staining in BTCC tumors and adjacentnoncancerous tissues were calculated. All the results were carried on statistics analysis.Results (1) The expression of MIF showed yellow or brown-yellow granular in BTCCand mainly located in the cytoplasm of cancer cells and a small quantity in themembrane, and MIF positive staining were significantly stronger in BTCC tumors thanthat in adjacent noncancerous tissues. The difference was statistically significant(P＜0.05), moreover related to lymph node metastasis, recurrent, pathological grade andclinical stage.(P＜0.05). The expression of VEGF showed homogeneous yellow orbrown-yellow granular mainly located in the cytoplasm of cancer cells next in thevascular endothelial cell and membrane, and VEGF positive staining were significantlystronger in BTCC tumors than that in adjacent noncancerous tissues. The difference wasstatistically significant(P＜0.05), moreover related to lymph node metastasis, recurrent, pathological grade and clinical stage.(P＜0.05). MIF mRNA and VEGF mRNAexpression level in BTCC group were higher than in normal bladder mucous membranetissues (P <0.05), and the amount of mRNA expression in invasive carcinoma tissuecompared with shallow carcinoma group. The difference was statistically significant (P<0.05).(2) The expression of CD31positive mainly locate in capillary endothelial cellnucleus, nucleus dyeing performance as tan grains, while CD34marked MVD in BTCCwere significantly higher than that in adjacent noncancerous tissues(P＜0.05). MIF、VEGF positive staining group MVD were significantly higher in BTCC tumors thanthat in adjacent noncancerous tissues, the difference was statistically significant(P＜0.05).(3) MIF、VEGF positive staining group MVD were significantly higher in BTCCtumors than that in adjacent noncancerous tissues, the difference was statisticallysignificant(P＜0.05). The expression of MIF and VEGF was significantly positivecorrelation by the Spearman correlation analysis, the difference was statisticallysignificant(P＜0.05).Conclusion (1) Along with the tumor grading increased, MIF, MIF mRNA expressionincreased significantly, moreover associated with the transfer of BTCC, recurrence,clinical classification and stage, at the same time, MIF expression and MVD werepositively correlated, expose that high expression of MIF may related construction oftumor vasculature, promote tumor angiogenesis, it could be a marker of tumor growth,recurrence, metastasis and prognosis.(2) Along with the tumor grading increased, theexpression VEGF、VEGF mRNA also increased significantly, moreover associated withthe transfer of BTCC, recurrence, clinical classification and stage, at the same time,MIF expression and MVD were positively correlated, VEGF may play an important rolein angiogenesis of BTCC, it could be a marker of tumor growth, recurrence, metastasisand prognosis.(3) The expression of MIF、VEGF not only increased along with thetumor grading increased, but also positively correlated with MVD. MIF and VEGF may have synergy in the process of tumor angiogenesis. And MIF positive staining grouporganization, the expression of VEGF and MVD were significantly increased, theanalysis showed that MIF was positively related with the two relations. Therefore, MIFmay promote the expression of VEGF in BTCC tissue, so as to achieve indirectlypromote the role of tumor angiogenesis.