Effect Of Carbon Dioxide Pneumoperitoneum On Serum Levels Of IL-1, IL-6, CD11a/CD18 And CD11b/CD18 And Pancreatic Pathology In Severe Acute Pancreatitis

Objective To examine the effect of carbon dioxide pneumoperitoneum on local pancreas pathological changes, serum levels of amylase, IL-1, IL-6, and the positive rate of dissolubility adhesion molecule (CD11a/CD18 and CD11b/CD18) in rats with severe acute pancreatitis (SAP).Methods Fifty healthy male SD rats were randomly divided into 3 groups: CO₂ pneumoperitoneum group (n=20): SAP was induced by injecting 5% sodium taurocholate through retrogradely common biliopancreatic ducts via duodenal papilla,and then CO₂ pneumoperitoneum was established at a pressure of 12 mmHg for 30 min; SAP group (n=20): The rats were treated as same as CO₂ pneumoperitoneum group, except CO₂ pneumoperitoneum;Simple operation group (n=10): Laparotomy was performed and nothing was done to duodenum and pancreas except for moving them softly. The blood samples were collected for examining serum levels of amylase, IL-1, IL-6, and the positive rates of CD11a/CD18 and CD11b/CD18, and histopathologic examination of pancreas was performed.Results Compared with simple operation group, the pancreatic pathologic histology score, serum levels of amylase, IL-1, IL-6, and the positive rates of CD11a/CD18 and CD11b/CD18 expression were significantly higher in CO₂ pneumoperitoneum group and SAP group(P<0.05), The level of IL-1 and IL-6 were significantly lower in CO₂ pneumoperitoneum group as compared to SAP group(P<0.05). There was no significant difference between CO₂ pneumoperitoneum group and SAP group in pancreatic pathologic histology score(P=0.294), the levels of serum amylase(P=0.073), the positive rates of CD11a/CD18(P=0.155) and CD11b/CD18 expression (P=0.201).Conclusion CO₂ pneumoperitoneum has inhibitory effect on the levels of IL-1 and IL-6, rather than the positive rates of CD11a/CD18 and CD11b/CD18 expression in SD rats with SAP.