| Objective: To evaluate the dispersion stability and transfection efficiency of hydroxyapatitie(HA) nanoparticles as Gene Transfer Carrier,which was surface modified by polyethyleneimine surface,and to lay the theoretical foundation for how to enhance the dispersion stability and transfection efficiency of hydroxyapatit- ie (HA) nanoparticles.Methods: polyethyleneimine solution was added into HA suspension for surface modification,The composite morphology and particle size of nHA-PEI nanoparticles were observed by transmission electron microscope (TEM). The ability of modified HA nanoparticles in absorbing and protecting DNA, and the way of nHAP-PEI/DNA dissociation were analyzed by agarose gel electrophoresis analysis and ultraviolet spectrophotometer. The potential of nHA-PEI and HA in acid, alkaliand neutral conditions were measured by Zeta Potential Instrument. MTT assy was performed to evaluate the toxicity of nHAP-PEI(10KD) to HepG2 cells. The combinations of enhanced green fluorescent protein plasmid pEGFP1 and nHA-PEI were transfected into HepG2, Hela and SW620 cells, and the transfection efficiency was evaluated by Flow Cytometry.Results: nHAP-PEI (10KD) are in high dispersion and the size of particle is 60 -80nm. At PH of 7.2, Zeta Potential of 42.87mV, The load of gene carrier of HA with chitosan surface modification was higher than that of HA.The modified HA nanoparticle had the ability of carrying and protecting DNA,The absorbed DNA can be washed off by PB solution,nHAP-PEI transfects into experiment cells, especially in HepG2 cells, followed by Hela, SW620.The transfection efficiency of nHAP-PEI was higher than PEI (10)and nHA, but lower than liposomes.Conclusions: The modified HA by PEI cation can potentiate the transfection of enhanced green fluorescent protein plasmid into HepG2 cells. nHAP-PEI (10KD) composite nanoparticles are expected to become an effective gene delivery vector.
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