The Experimental Study On The Effect Of The Content Of Nogo-A Protein And MAG In Rabbit's Sciatic Nerve Injury And Repairment By Acupuncture Treatment

Objective: To observe the therapeutic effect of treating peripheral nerve injury with acupuncture and provide new theoretical basis for clinic by experimental research of Nogo-A protein and myelin-associated glycoprotein(MAG) after sciatic nerve injury(SNI) in rabbits.Methods: 48 healthy adult rabbits were randomly divided into 3 groups: blank control group, model control group and acupuncture treatment group. Except blank control group, the right sciatic nerve injury(SNI) model of SunderlandⅡ～Ⅲdegree were made in other two groups by operation .One day after operation, we punctured Huantiao(GB 30) and Zusanli(ST 36) of the affected side in EA group, and applied even reinforcing-reducing by twirling-rotating manipulation, twisted it for 30 seconds every time and moved it one time per 10 minutes, retained the needle for 20 minutes, once daily, seven treatments made a course and there was one day break between two courses. The model control group was grasped every day but with no treatment. We observed the function of posterior right paw of all animals and examined the content of Nogo-A protein and MAG in the corresponding spinal cord segment.Results:(1) After finishing the treatment, Function of injured limbs were obviously improved acupuncture treatment group. (2) After a therapeutic course, the content of Nogo-A protein in acupuncture treatment group were higher than that of blank control group while lower than model control group, there was significant difference (P<0.05); two courses later, the content of Nogo-A protein in acupuncture treatment group was significantly lower than that of model control group(P<0.05) and there was no significant difference with blank control group (P>0.05). (3)The content of MAG in anterior horn of spinal cord: a therapeutic course later there was no significant difference between acupuncture treatment group and model control group(P > 0.05), while significantly higher than that of model control group(P<0.05); after treating for 14 days, the content of MAG in acupuncture treatment group was lower than that of that in model control group, there was a significant difference between groups (P<0.05) ,while remarkably higher than that of blank control group(P<0.05).Conclusion: Our research showed that acupuncture treatment could improve the functional recovery of acute SNI in rabbits, restrain the release of inhibitory growth factor in spinal cord, promote the growth of axon and the regeneration of affected nerve.