| Hormones are component of organic matters which are effective for human or animals. Hormone drugs include: glucocorticoid, adrenocorticotropic, norepinephrine, progesterone, estrogen, androgen, et al. Their common usage are intravenous, oral, topical and others. Common dosage forms include injection of powder, water injection, capsules, tablets, creams, pastes, aerosols. Hormones can be used to prevent, treat, diagnose disease and help the body return to normal function. For example, glucocorticoids mainly affects sugar and protein metabolism, and can fight inflammation.Due to its high sensitivity, simplicity and relatively low cost, chemiluminescence analysis has been widely used in biological engineering, molecular biologic, medicine, environmental chemistry.The thesis consists of two parts.The first one is a brief review dealing with the research and development of chemiluminescence. The other is research reports.In Chapter two, a nivel flow injection chemiluminescence method for the determination of prednisone acetate was developed. This method is based on that the strong CL of N-bromosuccinimide (NBS) and calcein can be inhibited by prednisone acetate. The CL intensity correlated linearly with the concentration of prednisone acetate over the range of 2.0×10-8~1.0×10-5 g/mL, the detection limit (3σ) of prednisone acetate was 1.3×10-8 g/mL. This method was applied to the determination of prednisone acetate in the tablets with good results. The mechanisms of CL reaction were also studied.In Chapter three, a nivel flow injection chemiluminescence method for the determination of estradiol was developed. This method is based on that the strong CL of N-bromosuccinimide (NBS) and calcein can be inhibited by estradiol. The CL intensity correlated linearly with the concentration of estradiol over the ranges of 2.0×10-8~8.0×10-6 g/mL. The detection limit (3σ) of estradiol was 8.5×10-9 g/mL. This method was applied to the determination of estradiol in the injections. The mechanisms of CL reaction were also studied.In Chapter four, a novel chemiluminescence (CL) method for the fast, simple and sensitive determination of estrogen benzoate using the reaction of tris (1,10-phenanthroline) ruthenium (II)-Na2SO3-permanganate is described. This method is based on the CL reaction of estrogen benzoate (EB) with acidic potassium permanganate and tris (1,10-phenanthroline) ruthenium (II). The CL intensity is greatly enhanced when Na2SO3 is added. After optimization of the different experimental parameters, a calibration graph for estrogen benzoate is linear in the range of 5.0×10-8~1.0×10-5 g/mL. The limits (3σ) of detection is 2.4×10-8 g/mL and the relative standard deviation was 1.3 % for 1.0×10-6 g/mL estrogen benzoate (n = 11). This proposed method was successfully applied to commercial injection samples. The mechanism of CL reaction was also studied.In Chapter five, a new chemiluminescence method is proposed for the determination of progesterone, which is based on the reaction of KMnO4- NaSO3 progesterone system. Under the optimum conditions, the linear range of progesterone is 8.0×10-8~1.0×10-6 g/mL. The detection limit (3σ) is 4.6×10-8 g/mL for progesterone.In Chapter six, to describe a novel flow injection chemiluminescence method for the determination of estradiol. The method is based on the oxidation of estradiol by acidic permanganate solution to give weak CL signal, which is dramatically enhanced by rhodamine B. The CL signal is linear with estradiol concentration in the range 2.0×10-8~1.0×10-5 g/mL with a detection limit of 1.2×10-8 g/mL (3σ). The method has been applied to the determination of estradiol in pharmaceutical preparations.
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