| The process of radiation produced in a chemical reaction from the decay of an excited state to the ground state is known as chemiluminescence (CL). Flow-injection analysis (FIA) is a technology in non-equilibrium thermodynamic conditions which the sample or reagent in the carrier reappearing in the handling zone. Combined with chemiluminescence and flow injection form a new method, it has many features as high sensitivity, wide linear range, quick analysis, simple and easy to implement automation equipment. It has been widely used in biomedicine, environmental monitoring, food hygiene and materials areas. This article is based on the drugs has enhancement or inhibition the luminol system, mainly studies carried out in the following areas:1.Studied in alkaline medium, cefradine could enhanced the intensity of luminol - H2O2 chemiluminescence system, established a new flow injection chemiluminesce- nce method determination of cefradine with simple, sensitive and stable features. In the optimum conditions, the concentration of cefradine showed good linear relationsh- ip in the range of 1.0×10-8 1.0×10-6 g/L with a detection limit of 6.1×10-11 g/L, and the relative standard deviation (RSD) was 1.9% (n=11,Ï=1.0×10-8 g/L). This method was applied to determine cefradine in the capsules, and the recoveries were between 98.8% 103.2%.2.Studied in alkaline medium, ampicillin sodium enhanced luminol-potassium ferricyanide system, established a new flow injection chemiluminescence method to detect ampicillin sodium. In the optimum conditions, the concentration of ampicillin sodium showed good linear relationship in the range of 5.0×10-6 1.0×10-3 g/L with a detection limit of 4.9×10-6 g/L, and the relative standard deviation (RSD) was 2.1% (n=11,Ï=5.0×10-4 g/L). This method was applied to determine ampicillin sodium in the injection, and the recoveries were between 98.4% 101.2%.3.A novel chemiluminescence method coupled with flow injection technique for the determination of cefoperazone sodium was developed. It was based on the fact that cefoperazone sodium could enhance chemiluminescence intensity when hemoglo- bin was used as a mimetic peroxidase in the catalytic chemiluminescence reaction of luminol and hydrogen peroxidase. In the optimum experimental conditions, the concentration of cefoperazone sodium showed good linear relationship in the range of 5.0×10-6 1.0×10-3 g/L with a detection limit of 2.6×10-6 g/L, and the relative standard deviation (RSD) was1.3% (n=11,Ï=4.0×10-5 g/L). This method was applied to determine cefoperazone sodium in the injection, and the recovery ratio was between 97.6% 106.2%.
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