Expression Of VEGF MRNA Induced By Angiotensin Ⅱ In Human Hepatic Cancer Cell Line HepG2

Objective:To investigate the effects of Angiotensin (Ang)â…¡on the expression of vascular endothelial growth factor (VEGF) in human hepatic cancer cell line HepG2Methods:Cultured HepG2 cells were treated by Angâ…¡with various concentrations and time which were collected at different time points. Then total RNA were extracted. The expression of VEGF mRNA in cultured HepG2 were determined by Semi-Quantitative reverse transcription polymerase chain reaction(RT-PCR), and the proliferation were detected by methyl thiazolyl tetrazolium (MTT).Results:Angâ…¡can stimulate the proliferation of HepG2 cells, and enhance the expression of VEGF mRNA (p<0.05), which was dose-and time-dependent. When the concentration of Angâ…¡is 10-7 mol/L, the time is 60h, the effect reaches the maximum value, which could be suppressed by Angiotensinâ…¡type 1 receptor (AT1R) antagonist.Conclusion:The expression of VEGF mRNA can be induced by Angâ…¡in human hepatic cancer cell line HepG2 through AT1R, which may play a definite role in the tumor growth and metastasis.