HPLC For Fingerprint Analysis Of Flavoneids Extracts In Ampelopsis Arossedntata

Ampelopsis grossedentata, also named MaoYanMei, Vine Tea, Dew Tea, White Tea, is a kind of wooden vine plant from Ampelopsis michx, Grape family. It mainly lives in GuangDong, YuNan, GuiZhou, HuNan, HuBei, JiangXi, FuJian provinces. Researches showed that it has medical functions like expelling toxin cooling, and active in hepatitis, flu, throat ache, arthritis, rheumatics. Recently, lots of domestic researches focus on the utilities of Vine Tea, and references show flavonids is rich contained in Vine Tea with reputation of highest volume of flavonids in those known plants. Attributing to its high value in medicine, ZhangJiajie, Hunan province has become largest base of Vine Tea farm; and the flavone compounds extraction is the pivotal industry in exploitation of Vine Tea.The research targeted on the flavonids extraction in the Vine Tea from ZhangJiajie, and clarified the technical process. Finger-print were figured out by HPLC, and antagonism of flavone compound were tested against sorts of microbes. Results show:1. Single factor and orthogonal experiments were used in optimizing the Heat-Backflow extraction craft of flavone, and optimum parameters were figured out. In the important comparation, extraction temperature>stuff liquid ratio>backflow times>extraction time. The optimum parameters were:extraction temperature 100℃, extraction time 45 min, stuff liquid ratio 100:1, backflow times 2, extraction efficiency 35.93%.2. Finger-print were figured out by HPLC, chromatograph requirements were:chromatographic column C18 (4.6 X 250mm), detective wavelength 270nm, column temperature 25℃, sample size 20μL, flow velocity 1.0mL/min, fluxion phase in gradient elution is acetonitrile-10% glacial acetic acid. Compounds in extraction were best separated by HPLC with the mentioned requirements, and the ratio error of relative peak square from 10 samples was within 10%, which proved the craft is stable and feasible.3. The antagonism effects were respectively tested between the flavone compound and Escherichia coli, Staphylococcus aurecus, Saccharomyces cerevisiae, Aspergillus niger. No antagonism was found between flavone compound with various mass concentrations and mould. While, apparent antagonisms were found between flavone compounds and bacteria like coccidia, bacillus, GNB and GPB. Flavone compound could control yeast to a certain extent and had lowest bacteriostatic concentration of 0.33g/L against bacillus subtilis and colibacillus, and lower than 0.17g/L against staphylococcus aureus, and respectively had disinfecting concentration of 1.29 g/L,0.65 g/L,0.33 g/L and 2.58 g/L against bacillus subtilis, colibacillus, staphylococcus aureus and mould.