The Study Of Human Papillomavirus (HPV) Type-Distribution In Cervix Of Women From Dingxiang County, Shanxi Province And Preparation Of Standard Samples Of HPV61, 83 And 84

Objective:Cervical cancer is one of most common cancer in women worldwide. Molecular epidemiologic evidence clearly indicates that HPV is the principal cause of invasive cervical cancer and cervical intraepithelial neoplasia. This study is aiming to determine HPV type-distribution in cervix of women from Dingxiang County, Shanxi Province. Meanwhile, we make standard samples of HPV61,83 and 84 containing MY fragments which are positive controls as investigating the prevalence of HPV61,83 and 84 of species 3 genus a family papillomavirus.Materials and Methods:During November of 2008 to February of 2009, a population-based cervical cancer screening was conducted in Dingxiang County of Shanxi Province, and 822 women aged 15-76 took part in the screening. HPV DNA type distribution in 817 cervical cell samples was detected using consensus primers MY09/11 and GP5+/6+. Amplified fragments of HPV61,83 and 84 obtained from HPV molecular epidemiologic survey of Shanxi Province using HPV consensus primers MY09/11 were cloned in pMD18-T vectors. And the plasmids were sequenced, then nucleotide sequences and amino acid sequences were analyzed by BioEdit biological soft.Results:The overall HPV prevalence of women aged 15-76 in Shanxi Province was 15.1%. Single type of HPV in infected women accounted for 87.0%, whereas more than two types of infected women accounted for 13.0%. The peak age for women infected HPV was'35-44'. The most common 4 types were HPV 16,58,33 and 18 in rural area of Shanxi Province. And the prevalence of HPV in this area was higher as the pathologic levels like normal cytology, LSIL, HSIL, and cervical carcinoma were elevated. In one hand, HPV61 and HPV83 isolates were consistent with reference strains U31793 and AF151983 in nucleotide sequences, however there were four mutations of nucleotide (C6760T, T6931C, T6951C and C6987A) in HPV84 isolate compared with reference strain AF293960, among which C6987A resulted in D441E. In the other hand, we mixed the plasmids containing MY fragments of three isolates with C33A genomes which were HPV negative cervical carcinoma cell lines. Then we found the amplified sensitivity of standard samples of HPV61 using primers MY09/11 was higher than that of HPV83 and 84.Conclusions:In one such high risk rural area, Shanxi Province, the most common HPV type was HPV16, as followed by HPV58,33 and 18. This data provided theoretical basis to make vaccines to prevent cervical cancer in China, especially in Shanxi Province. We have made standard samples of HPV61,83 and 84 containing MY fragments which were positive controls as investigating the prevalence of HPV61,83 and 84 of species 3 genus a family papillomavirus.